| Literature DB >> 16923077 |
Manuela Pardini1, Federico Giannoni, Carla Palma, Elisabetta Iona, Aurelio Cafaro, Lara Brunori, Monica Rinaldi, Vito Michele Fazio, Maria Elena Laguardia, Damiano Cosimo Carbonella, Mauro Magnani, Barbara Ensoli, Lanfranco Fattorini, Antonio Cassone.
Abstract
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.Entities:
Mesh：
Substances：
Year: 2006 PMID： 16923077 DOI： 10.1111/j.1574-6968.2006.00390.x
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742