BACKGROUND: Matrix metalloproteinases (MMP) modulate end-organ complications of acute pancreatitis, but the correlation between increased MMP production and histological severity of disease remains unclear. We examined the role of MMP and pancreas histology on experimental acute pancreatitis. METHODS: Forty male Wistar albino rats were subjected to cerulein-induced pancreatitis (8, 16, 24 and 32 h groups) or sham treatment. The animals were killed at different time points and pancreatic tissues were harvested to assess MMP (1, 2 and 9) activity and inflammatory changes. RESULTS: Compared with other groups, 8 h group had decreased tissue MMP-1 concentrations. MMP-9 concentrations were lower in 24-h and 32-h groups, as were histological severity scores. MMP-2 activity did not differ among groups. Pancreatitis was prominent in 8-h, 16-h and 24-h groups by means of histology. CONCLUSION: Induction of pancreatitis by cerulein altered pancreatic MMP levels in the early phase of inflammation. Inhibition of MMP-2 and MMP-9 paralleled histological scores. Therefore, MMP may have a predictive value to assess histological severity.
BACKGROUND: Matrix metalloproteinases (MMP) modulate end-organ complications of acute pancreatitis, but the correlation between increased MMP production and histological severity of disease remains unclear. We examined the role of MMP and pancreas histology on experimental acute pancreatitis. METHODS: Forty male Wistar albino rats were subjected to cerulein-induced pancreatitis (8, 16, 24 and 32 h groups) or sham treatment. The animals were killed at different time points and pancreatic tissues were harvested to assess MMP (1, 2 and 9) activity and inflammatory changes. RESULTS: Compared with other groups, 8 h group had decreased tissue MMP-1 concentrations. MMP-9 concentrations were lower in 24-h and 32-h groups, as were histological severity scores. MMP-2 activity did not differ among groups. Pancreatitis was prominent in 8-h, 16-h and 24-h groups by means of histology. CONCLUSION: Induction of pancreatitis by cerulein altered pancreatic MMP levels in the early phase of inflammation. Inhibition of MMP-2 and MMP-9 paralleled histological scores. Therefore, MMP may have a predictive value to assess histological severity.
Authors: David S Vitale; Patrick Lahni; Lindsey Hornung; Tyler Thompson; Peter R Farrell; Tom K Lin; Jaimie D Nathan; Hector R Wong; Maisam Abu-El-Haija Journal: PLoS One Date: 2022-02-14 Impact factor: 3.240
Authors: Felipe de Andrade Vieira Alves; Lucca de Lima S Oliveira; Natália Gedeão Salomão; David William Provance; Carlos Alberto Basilio-de-Oliveira; Rodrigo Basílio-de-Oliveira; Leandro Junqueira Moragas; Jorge José de Carvalho; Ronaldo Mohana-Borges; Kíssila Rabelo; Marciano Viana Paes Journal: PLoS One Date: 2022-01-18 Impact factor: 3.240