Literature DB >> 1691916

Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge.

K J Reddy1, R Webb, L A Sherman.   

Abstract

A procedure for the rapid preparation of cesium-chloride purified RNA from E. coli and the cyanobacterium Synechococcus sp. PCC7942 is described. Cells are lysed in modified sucrose, Triton X-100, EDTA, Tris buffer with phenol/chloroform. The cleared lysate is extracted further with phenol/chloroform and RNA is peleted by centrifugation through a 5.7 M CsCl cushion. High quality RNA can be prepared in three hours using this procedure.

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Year:  1990        PMID： 1691916

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  30 in total

1.  Identification of iron-responsive, differential gene expression in the cyanobacterium Synechocystis sp. strain PCC 6803 with a customized amplification library.

Authors:  A K Singh; L A Sherman
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

2.  Light-regulated expression of the psbD gene family in Synechococcus sp. strain PCC 7942: evidence for the role of duplicated psbD genes in cyanobacteria.

Authors:  S A Bustos; S S Golden
Journal:  Mol Gen Genet       Date:  1992-03

3.  Transcriptional and translational regulation of nitrogenase in light-dark- and continuous-light-grown cultures of the unicellular cyanobacterium Cyanothece sp. strain ATCC 51142.

Authors:  M S Colón-López; D M Sherman; L A Sherman
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

4.  The marC gene of Escherichia coli is not involved in multiple antibiotic resistance.

Authors:  Patrick F McDermott; Laura M McMurry; Isabelle Podglajen; JoAnn L Dzink-Fox; Thamarai Schneiders; Michael P Draper; Stuart B Levy
Journal:  Antimicrob Agents Chemother       Date:  2007-10-22       Impact factor: 5.191

5.  Role of sigma factors in controlling global gene expression in light/dark transitions in the cyanobacterium Synechocystis sp. strain PCC 6803.

Authors:  Tina C Summerfield; Louis A Sherman
Journal:  J Bacteriol       Date:  2007-08-24       Impact factor: 3.490

6.  Global transcriptional response of the alkali-tolerant cyanobacterium Synechocystis sp. strain PCC 6803 to a pH 10 environment.

Authors:  Tina C Summerfield; Louis A Sherman
Journal:  Appl Environ Microbiol       Date:  2008-07-07       Impact factor: 4.792

7.  The stpA gene form synechocystis sp. strain PCC 6803 encodes the glucosylglycerol-phosphate phosphatase involved in cyanobacterial osmotic response to salt shock.

Authors:  M Hagemann; A Schoor; R Jeanjean; E Zuther; F Joset
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

8.  Regulation and sequence of the Synechococcus sp. strain PCC 7942 groESL operon, encoding a cyanobacterial chaperonin.

Authors:  R Webb; K J Reddy; L A Sherman
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

9.  The mechanism of iron homeostasis in the unicellular cyanobacterium synechocystis sp. PCC 6803 and its relationship to oxidative stress.

Authors:  Sigal Shcolnick; Tina C Summerfield; Lilia Reytman; Louis A Sherman; Nir Keren
Journal:  Plant Physiol       Date:  2009-06-26       Impact factor: 8.340

10.  Regulation of Actinobacillus actinomycetemcomitans leukotoxin expression: analysis of the promoter regions of leukotoxic and minimally leukotoxic strains.

Authors:  J M Brogan; E T Lally; K Poulsen; M Kilian; D R Demuth
Journal:  Infect Immun       Date:  1994-02       Impact factor: 3.441
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