Interferon treatment reduces endocytosis of virus and facultatively intracellular bacteria in various cell lines.

Previous studies have shown that interferons (IFNs) specifically interact with a number of cells cultured in vitro and reduce the invasiveness of facultatively intracellular bacteria. IFN treatment also reduced the internalization of vesicular stomatitis virus (VSV) in cell cultures. Here we show that the anti-invasive effect of IFN on bacteria is eliminated in an L-cell variant where its effect on the uptake of vesicular stomatitis virus is lost. The data strongly suggest that the anti-invasive effect of IFN is mediated through inhibition of endocytosis.