Use of terpene-based products, with and without anhydrous alcohol, as clearing agents in trichrome staining technique for intestinal protozoa.

BDH xylene substitute, a terpene-based product, and its mixture with anhydrous alcohol were found to be excellent replacements for xylene and carbol-xylene, respectively, in the trichrome staining technique applied to sodium acetate-acetic acid-Formalin-fixed fecal smears for detection of intestinal protozoa.