Literature DB >> 1690956

Purification of gramicidin A.

C J Stankovic1, J M Delfino, S L Schreiber.   

Abstract

A simple chromatographic purification of the naturally occurring ion channel-forming pentadecapeptide gramicidin A (gA) is presented. This procedure allows gA to be isolated in gram quantities from the commercially available mixture of isomers after chromatography on silica gel. The gramicidin A obtained in this manner is greater than 95% pure as determined by 1HNMR, HPLC, and amino acid analysis.

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Year:  1990        PMID: 1690956     DOI: 10.1016/0003-2697(90)90019-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  7 in total

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Authors:  Sheereen Majd; Erik C Yusko; Jerry Yang; David Sept; Michael Mayer
Journal:  Biophys J       Date:  2013-07-02       Impact factor: 4.033

2.  Gramicidin pores report the activity of membrane-active enzymes.

Authors:  Sheereen Majd; Erik C Yusko; Alexander D MacBriar; Jerry Yang; Michael Mayer
Journal:  J Am Chem Soc       Date:  2009-11-11       Impact factor: 15.419

3.  A semi-synthetic ion channel platform for detection of phosphatase and protease activity.

Authors:  Michael X Macrae; Steven Blake; Xiayun Jiang; Ricardo Capone; Daniel J Estes; Michael Mayer; Jerry Yang
Journal:  ACS Nano       Date:  2009-11-24       Impact factor: 15.881

Review 4.  Model ion channels: gramicidin and alamethicin.

Authors:  G A Woolley; B A Wallace
Journal:  J Membr Biol       Date:  1992-08       Impact factor: 1.843

5.  The structure of an integral membrane peptide: a deuterium NMR study of gramicidin.

Authors:  R S Prosser; S I Daleman; J H Davis
Journal:  Biophys J       Date:  1994-05       Impact factor: 4.033

6.  Membrane-mediated action of the endocannabinoid anandamide on membrane proteins: implications for understanding the receptor-independent mechanism.

Authors:  Djalma Medeiros; Laíz da Costa Silva-Gonçalves; Annielle Mendes Brito da Silva; Marcia Perez Dos Santos Cabrera; Manoel Arcisio-Miranda
Journal:  Sci Rep       Date:  2017-01-27       Impact factor: 4.379

7.  Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi.

Authors:  André L A Souza; Robson X Faria; Kátia S Calabrese; Daiane J Hardoim; Noemi Taniwaki; Luiz A Alves; Salvatore G De Simone
Journal:  PLoS One       Date:  2016-07-06       Impact factor: 3.240

  7 in total

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