| Literature DB >> 16908184 |
Charles J Heise1, Melanie H Cobb.
Abstract
Mitogen-activated protein kinases (MAPKs) are common signal transducers in all eukaryotic organisms. MAPKs are activated by protein kinase cascades consisting of MAPK kinases (MAP2Ks) and MAPK kinase kinases (MAP3Ks). Extracellular-signal regulated kinases 1 and 2 (ERK1/2) are the best characterized MAPKs. Like other MAPKs their activity is regulated by dual phosphorylation as well as dephosphorylation by a host of phosphoprotein phosphatases. The ability to phosphorylate or thiophosphorylate ERK2 in vitro, as described here, is valuable for use in downstream applications designed to investigate MAPK signaling networks.Entities:
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Year: 2006 PMID： 16908184 DOI： 10.1016/j.ymeth.2006.06.012
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608