| Literature DB >> 16906143 |
Ingo H Gorr1, Alexandra Reis, Dominik Boos, Martin Wühr, Suzanne Madgwick, Keith T Jones, Olaf Stemmann.
Abstract
Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.Entities:
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Year: 2006 PMID： 16906143 PMCID： PMC2435240 DOI： 10.1038/ncb1467
Source DB: PubMed Journal: Nat Cell Biol ISSN： 1465-7392 Impact factor: 28.824