Literature DB >> 1690519

Human cardiac sodium channels expressed in Xenopus oocytes.

G F Tomaselli1, A M Feldman, G Yellen, E Marban.   

Abstract

We report the expression of voltage-dependent Na+ channels in Xenopus oocytes injected with total RNA isolated from explanted human hearts. The expressed channels demonstrate characteristic voltage-dependent gating, inhibition by tetrodotoxin, and selectivity for Na+. Oocytes injected with sterile water or intentionally degraded RNA had no similar channel activity. The antiarrhythmic agent lidocaine (20 microM) inhibits current flow through the channel in a voltage-dependent fashion. Na+ channels expressed by injection of human cardiac RNA into Xenopus oocytes qualitatively resemble channels in the native tissue.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1690519     DOI： 10.1152/ajpheart.1990.258.3.H903

Source DB:  PubMed          Journal:  Am J Physiol        ISSN： 0002-9513


  4 in total

1.  Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel.

Authors:  M E Gellens; A L George; L Q Chen; M Chahine; R Horn; R L Barchi; R G Kallen
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

2.  Simultaneous expression of cardiac and skeletal muscle isoforms of the L-type Ca2+ channel in a rat heart muscle cell line.

Authors:  R Mejía-Alvarez; G F Tomaselli; E Marban
Journal:  J Physiol       Date:  1994-07-15       Impact factor: 5.182

3.  Mutations affecting agonist sensitivity of the nicotinic acetylcholine receptor.

Authors:  G F Tomaselli; J T McLaughlin; M E Jurman; E Hawrot; G Yellen
Journal:  Biophys J       Date:  1991-09       Impact factor: 4.033

4.  Expression of cardiac Na channels with appropriate physiological and pharmacological properties in Xenopus oocytes.

Authors:  D S Krafte; W A Volberg; K Dillon; A M Ezrin
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.