Literature DB >> 16898780

Click chemistry as a reliable method for the high-density postsynthetic functionalization of alkyne-modified DNA.

Johannes Gierlich1, Glenn A Burley, Philipp M E Gramlich, David M Hammond, Thomas Carell.   

Abstract

[reaction: see text] We report the development of the Cu(I)-catalyzed Huisgen cycloaddition (click) reaction for the multiple postsynthetic labeling of alkyne-modified DNA. A series of alkyne-modified oligodeoxyribonucleotides (ODNs) of increasing alkyne density were prepared, and the click reaction using various azide labels was investigated. Complete high-density conversion was observed for ODNs containing up to six consecutive alkyne functions. Compatibility of the click conditions with long DNA strands was shown using a PCR product obtained with an alkyne-modified primer.

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Year:  2006        PMID： 16898780     DOI： 10.1021/ol0610946

Source DB:  PubMed          Journal:  Org Lett        ISSN： 1523-7052            Impact factor:   6.005


  63 in total

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4.  Targeting Base Excision Repair Glycosylases with DNA Containing Transition State Mimics Prepared via Click Chemistry.

Authors:  Philip K Yuen; Sydnee A Green; Jonathan Ashby; Kori T Lay; Abhishek Santra; Xi Chen; Martin P Horvath; Sheila S David
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Journal:  Bioconjug Chem       Date:  2011-07-22       Impact factor: 4.774

8.  Visualizing metabolically labeled glycoconjugates of living cells by copper-free and fast huisgen cycloadditions.

Authors:  Xinghai Ning; Jun Guo; Margreet A Wolfert; Geert-Jan Boons
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10.  Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation.

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Journal:  Electrophoresis       Date:  2014-07-24       Impact factor: 3.535
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