| Literature DB >> 16898226 |
Atsushi Miki1, Michiki Narushima, Teru Okitsu, Yuichi Takeno, Alejandro Soto-Gutierrez, Jorge David Rivas-Carrillo, Nalú Navarro-Alvarez, Yong Chen, Kimiaki Tanaka, Hirofumi Noguchi, Shinichi Matsumoto, Michinori Kohara, Jonathan R T Lakey, Eiji Kobayashi, Noriaki Tanaka, Naoya Kobayashi.
Abstract
Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for alpha-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.Entities:
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Year: 2006 PMID： 16898226
Source DB: PubMed Journal: Cell Transplant ISSN： 0963-6897 Impact factor: 4.064