Development of simple and sensitive spectrophotometric method for the determination of bendiocarb in its formulations and environmental samples.

Facile, selective and sensitive spectrophotometric method has been developed for the determination of bendiocarb in its insecticidal formulations, fortified water, food grains, agriculture wastewater and agriculture soil samples with prepared reagents. The method was based on alkaline hydrolysis of the bendiocarb pesticide, and the resultant hydrolysis product of bendiocarb was coupled with 2,6-dibromo-4-methylaniline to give a yellow color product with lambda max of 457 nm or coupling with 2, 6-dibromo-4-nitroaniline to produce a red colored product with lambda max of 474 nm or coupling with 2, 4, 6-tribromoaniline to form orange red colored product has a lambda max of 465 nm. Under optimal conditions, Beer's law range for 2, 6-dibromo-4-methylaniline (DBMA) was found to be 0.6-14.0 microg mL (-1), 0.8-10.0 microg mL (-1) for 2, 6-dibromo-4-nitroaniline (DBNA) and 0.4-10.0 microg mL (-1) for 2, 4, 6-tribromoaniline (TBA). The molar absorptivity of the color systems were found to be 4.126 x 10(4) l mol (-1) cm (-1) for DBMA, 3.254 x 10(4) l mol (-1) cm (-1) for DBNA and 2.812 x 10(4) l mol (-1) cm (-1) for TBA. Sandell's of the color reactions are 0.018 microg cm (-2)(DBMA), 0.052 microg cm (-2)(DBNA) and 0.065 microg cm (-2) (TBA) respectively. The effect of the non-target species on the determination of bendiocarb was studied. The formation of colored derivatives with the coupling agents is instantaneous and stable for 18 h, 30 h, and 12 h. Performance of the proposed methods were compared statistically in terms Student's F and t-tests with the reported methods.