Fumihiko Mori1,2, Junichi Takahashi3, Taiji Nagaoka3, Toru Abiko3, Taiichi Hikichi3, Akitoshi Yoshida3. 1. Department of Ophthalmology, Hakodate Goryokaku Hospital, Hakodate, Japan. fmorichan@yahoo.co.jp. 2. Department of Ophthalmology, Asahikawa Medical College, Asahikawa, Japan. fmorichan@yahoo.co.jp. 3. Department of Ophthalmology, Asahikawa Medical College, Asahikawa, Japan.
Abstract
PURPOSE: To evaluate quantitatively the effects of bucillamine on the entrapment of leukocytes in the retinal microcirculation of diabetic rats. METHODS: 13 male Brown Norway rats were injected with streptozotocin (STZ). After the animals developed diabetes, they were divided into two groups. Group 1 (n=7) received fresh drinking water without bucillamine, and group 2 (n=6) received fresh drinking water supplemented with bucillamine (200 mg/kg per day). Rats that were not injected with STZ and received water without bucillamine served as controls (n=6). Four weeks after the injection of STZ, the leukocytes in the retina were observed by acridine orange digital fluorography. The number of leukocytes trapped in the retinal vessels was compared among the three groups. RESULTS: In the untreated diabetic rats, the number of trapped leukocytes was significantly higher than in control rats or bucillamine-treated diabetic rats (P<0.05). CONCLUSIONS: We demonstrated an inhibitory effect of bucillamine on leukocyte entrapment in the retinal vessels of diabetic rats. Bucillamine may have therapeutic efficacy in preventing the development of diabetic retinopathy. Copyright (c) Japanese Ophthalmological Society 2006.
PURPOSE: To evaluate quantitatively the effects of bucillamine on the entrapment of leukocytes in the retinal microcirculation of diabeticrats. METHODS: 13 male Brown Norway rats were injected with streptozotocin (STZ). After the animals developed diabetes, they were divided into two groups. Group 1 (n=7) received fresh drinking water without bucillamine, and group 2 (n=6) received fresh drinking water supplemented with bucillamine (200 mg/kg per day). Rats that were not injected with STZ and received water without bucillamine served as controls (n=6). Four weeks after the injection of STZ, the leukocytes in the retina were observed by acridine orange digital fluorography. The number of leukocytes trapped in the retinal vessels was compared among the three groups. RESULTS: In the untreated diabeticrats, the number of trapped leukocytes was significantly higher than in control rats or bucillamine-treated diabeticrats (P<0.05). CONCLUSIONS: We demonstrated an inhibitory effect of bucillamine on leukocyte entrapment in the retinal vessels of diabeticrats. Bucillamine may have therapeutic efficacy in preventing the development of diabetic retinopathy. Copyright (c) Japanese Ophthalmological Society 2006.
Authors: M Nagashima; K Wauke; D Hirano; S Ishigami; H Aono; M Takai; M Sasano; S Yoshino Journal: Rheumatology (Oxford) Date: 2000-11 Impact factor: 7.580
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