Literature DB >> 16897058

Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene products.

Steven D Buckingham1.   

Abstract

The selective knockdown of genes using RNA interference, and the analysis of the resultant phenotype using calcium imaging, is proving to be a powerful combination for discovering new genes involved in calcium signalling. This technical note describes the application of this approach to the Drosophila S2 cell line and its derivatives.

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Year:  2006        PMID： 16897058     DOI： 10.1007/s10158-006-0025-z

Source DB:  PubMed          Journal:  Invert Neurosci        ISSN： 1354-2516


  24 in total

1.  Characterization of low-glycosylated forms of soluble human urokinase receptor expressed in Drosophila Schneider 2 cells after deletion of glycosylation-sites.

Authors:  Henrik Gårdsvoll; Finn Werner; Leif Søndergaard; Keld Danø; Michael Ploug
Journal:  Protein Expr Purif       Date:  2004-04       Impact factor: 1.650

2.  Rho-kinase controls cell shape changes during cytokinesis.

Authors:  Gilles R X Hickson; Arnaud Echard; Patrick H O'Farrell
Journal:  Curr Biol       Date:  2006-02-21       Impact factor: 10.834

3.  Dbeta3, an atypical nicotinic acetylcholine receptor subunit from Drosophila : molecular cloning, heterologous expression and coassembly.

Authors:  Stuart J Lansdell; Neil S Millar
Journal:  J Neurochem       Date:  2002-03       Impact factor: 5.372

4.  RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis.

Authors:  Cezary Wójcik; Mihiro Yano; George N DeMartino
Journal:  J Cell Sci       Date:  2003-12-02       Impact factor: 5.285

5.  Inhibition of apoptosis by Z-VAD-fmk in SMN-depleted S2 cells.

Authors:  Raju Ilangovan; William L Marshall; Yimin Hua; Jianhua Zhou
Journal:  J Biol Chem       Date:  2003-06-03       Impact factor: 5.157

6.  Identification of pathways regulating cell size and cell-cycle progression by RNAi.

Authors:  Mikael Björklund; Minna Taipale; Markku Varjosalo; Juha Saharinen; Juhani Lahdenperä; Jussi Taipale
Journal:  Nature       Date:  2006-02-23       Impact factor: 49.962

7.  Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line.

Authors:  N S Millar; S D Buckingham; D B Sattelle
Journal:  Proc Biol Sci       Date:  1994-12-22       Impact factor: 5.349

8.  STIM1, an essential and conserved component of store-operated Ca2+ channel function.

Authors:  Jack Roos; Paul J DiGregorio; Andriy V Yeromin; Kari Ohlsen; Maria Lioudyno; Shenyuan Zhang; Olga Safrina; J Ashot Kozak; Steven L Wagner; Michael D Cahalan; Gönül Veliçelebi; Kenneth A Stauderman
Journal:  J Cell Biol       Date:  2005-05-02       Impact factor: 10.539

9.  D-MEKK1, the Drosophila orthologue of mammalian MEKK4/MTK1, and Hemipterous/D-MKK7 mediate the activation of D-JNK by cadmium and arsenite in Schneider cells.

Authors:  Olga P Ryabinina; Ezhilkani Subbian; Mihail S Iordanov
Journal:  BMC Cell Biol       Date:  2006-02-01       Impact factor: 4.241

10.  Identification of Drosophila gene products required for phagocytosis of Candida albicans.

Authors:  Shannon L Stroschein-Stevenson; Edan Foley; Patrick H O'Farrell; Alexander D Johnson
Journal:  PLoS Biol       Date:  2005-12-20       Impact factor: 8.029
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