Literature DB >> 1689571

Lysosomal acid phosphatase is transported via endosomes to lysosomes.

Y Tanaka1, S Yano, K Okada, T Ishikawa, M Himeno, K Kato.   

Abstract

Involvement of endosomes in transport of newly synthesized acid phosphatase to lysosomes was investigated using the Golgi fraction (GF1 + 2), enriched in endosomes. The Golgi fraction (GF1 + 2) was prepared from the livers of rats given [35S]methionine and asialofetuin conjugated-horseradish peroxidase (HRP). Newly synthesized acid phosphatase in the endosomes containing internalized asialofetuin-HRP was measured as a loss of the detectable labeled enzyme after 3,3'-diaminobenzidine (DAB) and H2O2 reaction, due to formation of insoluble polymers which reduce protein antigenicity. With this procedure, acid phosphatase was all but undetectable in the Golgi fraction. Thus, newly synthesized acid phosphatase is apparently transported to lysosomes by endosomes.

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Year:  1990        PMID： 1689571     DOI： 10.1016/0006-291x(90)90990-5

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  5 in total

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Authors:  A Hasilik
Journal:  Experientia       Date:  1992-02-15

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Review 3.  Autophagy and other vacuolar protein degradation mechanisms.

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Journal:  Experientia       Date:  1992-02-15

4.  The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane.

Authors:  J Rohrer; A Schweizer; D Russell; S Kornfeld
Journal:  J Cell Biol       Date:  1996-02       Impact factor: 10.539

5.  Identification of Aph1, a phosphate-regulated, secreted, and vacuolar acid phosphatase in Cryptococcus neoformans.

Authors:  Sophie Lev; Ben Crossett; So Young Cha; Desmarini Desmarini; Cecilia Li; Methee Chayakulkeeree; Christabel F Wilson; P R Williamson; Tania C Sorrell; Julianne T Djordjevic
Journal:  mBio       Date:  2014-09-16       Impact factor: 7.867
  5 in total

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