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Literature DB >> 1689050

In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli.

Abstract

It is not yet clear how bacterial outer membrane proteins reach their correct destination after they are secreted across the cytoplasmic membrane. We show here that porin OmpF is secreted into the medium as a water-soluble monomeric protein by spheroplasts of Escherichia coli. Furthermore, this monomeric porin is taken up by cell envelope preparations or purified lipopolysaccharides in the presence of 0.03% Triton X-100 and is converted correctly into the mature trimeric conformation. These results appear to reproduce a part of the physiological export and targeting steps of this protein.

Entities: Chemical Species

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Year: 1990 PMID： 1689050 PMCID： PMC53342 DOI： 10.1073/pnas.87.2.743

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

Review 1. Molecular basis of bacterial outer membrane permeability.

Authors: H Nikaido; M Vaara
Journal: Microbiol Rev Date: 1985-03

2. Subunit structure of functional porin oligomers that form permeability channels in the other membrane of Escherichia coli.

Authors: T Nakae; J Ishii; M Tokunaga
Journal: J Biol Chem Date: 1979-03-10 Impact factor: 5.157

3. Outer membrane of gram-negative bacteria. XVIII. Electron microscopic studies on porin insertion sites and growth of cell surface of Salmonella typhimurium.

Authors: J Smit; H Nikaido
Journal: J Bacteriol Date: 1978-08 Impact factor: 3.490

4. Pleiotropic transport mutants of Escherichia coli lack porin, a major outer membrane protein.

Authors: P Bavoil; H Nikaido; K von Meyenburg
Journal: Mol Gen Genet Date: 1977-12-14

5. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.

Authors: J P Rosenbusch
Journal: J Biol Chem Date: 1974-12-25 Impact factor: 5.157

6. The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis.

Authors: D M Engelman; T A Steitz
Journal: Cell Date: 1981-02 Impact factor: 41.582

7. Translocation and assembly of outer membrance proteins of Escherichia coli. Selective accumulation of precursors and novel assembly intermediates caused by phenethyl alcohol.

Authors: S Halegoua; M Inouye
Journal: J Mol Biol Date: 1979-05-05 Impact factor: 5.469

8. Models for the structure of outer-membrane proteins of Escherichia coli derived from raman spectroscopy and prediction methods.

Authors: H Vogel; F Jähnig
Journal: J Mol Biol Date: 1986-07-20 Impact factor: 5.469

9. A water-soluble form of porin from the mitochondrial outer membrane of Neurospora crassa. Properties and relationship to the biosynthetic precursor form.

Authors: R Pfaller; H Freitag; M A Harmey; R Benz; W Neupert
Journal: J Biol Chem Date: 1985-07-05 Impact factor: 5.157

10. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.

Authors: G F Ames; E N Spudich; H Nikaido
Journal: J Bacteriol Date: 1974-02 Impact factor: 3.490

37 in total

1. Physicochemical evidence that Treponema pallidum TroA is a zinc-containing metalloprotein that lacks porin-like structure.

Authors: R K Deka; Y H Lee; K E Hagman; D Shevchenko; C A Lingwood; C A Hasemann; M V Norgard; J D Radolf
Journal: J Bacteriol Date: 1999-07 Impact factor: 3.490

Review 2. Export and assembly of bacterial outer membrane proteins.

Authors: J Tommassen; M Struyvé; H de Cock
Journal: Antonie Van Leeuwenhoek Date: 1992-02 Impact factor: 2.271

3. Arrangement of the translocator of the autotransporter adhesin involved in diffuse adherence on the bacterial surface.

Authors: Daniel Müller; Inga Benz; Damini Tapadar; Christian Buddenborg; Lilo Greune; M Alexander Schmidt
Journal: Infect Immun Date: 2005-07 Impact factor: 3.441

In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli.

Review 1. Molecular basis of bacterial outer membrane permeability.

2. Subunit structure of functional porin oligomers that form permeability channels in the other membrane of Escherichia coli.

3. Outer membrane of gram-negative bacteria. XVIII. Electron microscopic studies on porin insertion sites and growth of cell surface of Salmonella typhimurium.

4. Pleiotropic transport mutants of Escherichia coli lack porin, a major outer membrane protein.

5. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding.

6. The spontaneous insertion of proteins into and across membranes: the helical hairpin hypothesis.

7. Translocation and assembly of outer membrance proteins of Escherichia coli. Selective accumulation of precursors and novel assembly intermediates caused by phenethyl alcohol.

8. Models for the structure of outer-membrane proteins of Escherichia coli derived from raman spectroscopy and prediction methods.

9. A water-soluble form of porin from the mitochondrial outer membrane of Neurospora crassa. Properties and relationship to the biosynthetic precursor form.

10. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.

1. Physicochemical evidence that Treponema pallidum TroA is a zinc-containing metalloprotein that lacks porin-like structure.

Review 2. Export and assembly of bacterial outer membrane proteins.

3. Arrangement of the translocator of the autotransporter adhesin involved in diffuse adherence on the bacterial surface.

4. Physicochemical characterization of the reassembled dimer of an integral membrane protein OmpF porin.

5. Permissive sites and topology of an outer membrane protein with a reporter epitope.

6. Reassembly of an integral oligomeric membrane protein OmpF porin in n-octyl beta-D: -glucopyranoside-lipids mixtures.

7. Deletions or duplications in the BtuB protein affect its level in the outer membrane of Escherichia coli.

8. In vivo reconstitution of the FhuA transport protein of Escherichia coli K-12.

Review 9. Outer membrane proteins of pathogenic spirochetes.

10. Analysis of the structure and subcellular location of filamentous phage pIV.