Literature DB >> 1689045

Monoclonal antibodies to cerebellar pinceau terminals obtained after immunization with brain mRNA-injected Xenopus oocytes.

G Tigyi1, C Matute, R Miledi.   

Abstract

A method was developed to produce monoclonal antibodies to brain cell antigens by using Xenopus oocytes as immunological vectors. The method consists in injecting Xenopus oocytes with rat brain mRNA to express foreign proteins and using the oocytes for immunization. Immunizations were preceded by immunotolerization of mice to antigens of native oocyte membranes. With this approach we generated a set of monoclonal antibodies that are specific markers for the cerebellar "pinceau"--a unique complex synapse formed between basket cell terminals and the initial segment of the Purkinje cell axon. Our findings reveal an immunoreactivity highly localized at the pinceau and its late expression beginning at postnatal day 19 during cerebellar development.

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Year:  1990        PMID： 1689045      PMCID： PMC53298          DOI： 10.1073/pnas.87.2.528

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.

Authors:  A L de Blas; J Vitorica; P Friedrich
Journal:  J Neurosci       Date:  1988-02       Impact factor: 6.167

2.  Effect of particle size on labeling density for catalase in protein A-gold immunocytochemistry.

Authors:  S Yokota
Journal:  J Histochem Cytochem       Date:  1988-01       Impact factor: 2.479

3.  A Mab to a unique cerebellar neuron generated by immunosuppression and rapid immunization.

Authors:  S Hockfield
Journal:  Science       Date:  1987-07-03       Impact factor: 47.728

4.  Postnatal development of the cerebellar cortex in the rat. II. Phases in the maturation of Purkinje cells and of the molecular layer.

Authors:  J Altman
Journal:  J Comp Neurol       Date:  1972-08       Impact factor: 3.215

5.  Autoradiographic and histological studies of postnatal neurogenesis. 3. Dating the time of production and onset of differentiation of cerebellar microneurons in rats.

Authors:  J Altman
Journal:  J Comp Neurol       Date:  1969-07       Impact factor: 3.215

6.  Purification and characterization of P400 protein, a glycoprotein characteristic of Purkinje cell, from mouse cerebellum.

Authors:  N Maeda; M Niinobe; K Nakahira; K Mikoshiba
Journal:  J Neurochem       Date:  1988-12       Impact factor: 5.372

7.  Cyclophosphamide treatment used to manipulate the immune response for the production of monoclonal antibodies.

Authors:  W D Matthew; A W Sandrock
Journal:  J Immunol Methods       Date:  1987-06-26       Impact factor: 2.303

8.  Polyspecific natural antibodies and autoantibodies secreted by human lymphocytes immortalized with Epstein-Barr virus.

Authors:  J M Seigneurin; B Guilbert; M J Bourgeat; S Avrameas
Journal:  Blood       Date:  1988-03       Impact factor: 22.113

9.  Specificities and V genes encoding monoclonal autoantibodies from viable motheaten mice.

Authors:  C J Painter; M Monestier; A Chew; A Bona-Dimitriu; K Kasturi; C Bailey; V E Scott; C L Sidman; C A Bona
Journal:  J Exp Med       Date:  1988-03-01       Impact factor: 14.307

10.  Specialized membrane junctions between neurons in the vertebrate cerebellar cortex.

Authors:  C Sotelo; R Llinás
Journal:  J Cell Biol       Date:  1972-05       Impact factor: 10.539
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