BACKGROUND AND PURPOSE: Breast cancer is diagnosed worldwide in approximately one million women annually and radiation therapy is an integral part of treatment. The purpose of this study was to investigate the molecular basis underlying response to radiotherapy in breast cancer tissue. MATERIAL AND METHODS: Tumour biopsies were sampled before radiation and after 10 treatments (of 2 Gray (Gy) each) from 19 patients with breast cancer receiving radiation therapy. Gene expression microarray analyses were performed to identify in vivo radiation-responsive genes in tumours from patients diagnosed with breast cancer. The mutation status of the TP53 gene was determined by using direct sequencing. RESULTS AND CONCLUSION: Several genes involved in cell cycle regulation and DNA repair were found to be significantly induced by radiation treatment. Mutations were found in the TP53 gene in 39% of the tumours and the gene expression profiles observed seemed to be influenced by the TP53 mutation status.
BACKGROUND AND PURPOSE:Breast cancer is diagnosed worldwide in approximately one million women annually and radiation therapy is an integral part of treatment. The purpose of this study was to investigate the molecular basis underlying response to radiotherapy in breast cancer tissue. MATERIAL AND METHODS: Tumour biopsies were sampled before radiation and after 10 treatments (of 2 Gray (Gy) each) from 19 patients with breast cancer receiving radiation therapy. Gene expression microarray analyses were performed to identify in vivo radiation-responsive genes in tumours from patients diagnosed with breast cancer. The mutation status of the TP53 gene was determined by using direct sequencing. RESULTS AND CONCLUSION: Several genes involved in cell cycle regulation and DNA repair were found to be significantly induced by radiation treatment. Mutations were found in the TP53 gene in 39% of the tumours and the gene expression profiles observed seemed to be influenced by the TP53 mutation status.
Authors: Janet K Horton; Sharareh Siamakpour-Reihani; Chen-Ting Lee; Ying Zhou; Wei Chen; Joseph Geradts; Diane R Fels; Peter Hoang; Kathleen A Ashcraft; Jeff Groth; Hsiu-Ni Kung; Mark W Dewhirst; Jen-Tsan A Chi Journal: Radiat Res Date: 2015-10-21 Impact factor: 2.841
Authors: Ann Rita Halvorsen; Aslaug Helland; Thomas Fleischer; Karen Marie Haug; Grethe Irene Grenaker Alnaes; Daniel Nebdal; Randi G Syljuåsen; Nizar Touleimat; Florence Busato; Jörg Tost; Anna B Saetersdal; Anne-Lise Børresen-Dale; Vessela Kristensen; Hege Edvardsen Journal: Int J Cancer Date: 2014-04-05 Impact factor: 7.396
Authors: L Fabris; S Berton; F Citron; S D'Andrea; I Segatto; M S Nicoloso; S Massarut; J Armenia; G Zafarana; S Rossi; C Ivan; T Perin; J S Vaidya; M Avanzo; M Roncadin; M Schiappacassi; R G Bristow; G Calin; G Baldassarre; B Belletti Journal: Oncogene Date: 2016-02-15 Impact factor: 9.867