Literature DB >> 16890031

Quantitative analysis of s-adenosylmethionine and s-adenosylhomocysteine in neurulation-stage mouse embryos by liquid chromatography tandem mass spectrometry.

Katie A Burren1, Kevin Mills, Andrew J Copp, Nicholas D E Greene.   

Abstract

The potential importance of the methylation cycle during embryonic development necessitates the establishment of methodology to detect alterations in the relative abundance of s-adenosylmethionine (SAM) and s-adenosylhomocysteine (SAH) in an embryonic experimental system. We have developed a precise and sensitive method for measurement of SAM and SAH based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in single neurulation-stage mouse embryos. Use of a penta-fluorinated high-performance liquid chromatography (HPLC) stationary phase gave enhanced sensitivity due to optimal ionisation in organic mobile phase and increased retention time compared to standard reversed-phase separation. Calibration curves suitable for the analysis of neurulation-stage mouse embryos (SAM 0.02-25.0microM, SAH 0.01-10.0microM) were linear (r(2)>0.997) with limits of detection for SAM and SAH of 10 and 2.5nmol/L, respectively.

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Year:  2006        PMID： 16890031     DOI： 10.1016/j.jchromb.2006.07.012

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  12 in total

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Journal:  J Med Food       Date:  2014-09-30       Impact factor: 2.786

2.  The application of a chemical determination of N-homocysteinylation levels in developing mouse embryos: implication for folate responsive birth defects.

Authors:  Kristin Fathe; Maria D Person; Richard H Finnell
Journal:  J Nutr Biochem       Date:  2014-11-12       Impact factor: 6.048

3.  Neural tube defects induced by folate deficiency in mutant curly tail (Grhl3) embryos are associated with alteration in folate one-carbon metabolism but are unlikely to result from diminished methylation.

Authors:  Sandra C P De Castro; Kit-Yi Leung; Dawn Savery; Katie Burren; Rima Rozen; Andrew J Copp; Nicholas D E Greene
Journal:  Birth Defects Res A Clin Mol Teratol       Date:  2010-08

4.  Validated HPLC-Fl method for the analysis of S-adenosylmethionine and S-adenosylhomocysteine biomarkers in human blood.

Authors:  Camelia Albu; Simona Carmen Litescu; Gabriel Lucian Radu; Hassan Y Aboul-Enein
Journal:  J Fluoresc       Date:  2013-02-14       Impact factor: 2.217

5.  Gene-environment interactions in the causation of neural tube defects: folate deficiency increases susceptibility conferred by loss of Pax3 function.

Authors:  Katie A Burren; Dawn Savery; Valentina Massa; Robert M Kok; John M Scott; Henk J Blom; Andrew J Copp; Nicholas D E Greene
Journal:  Hum Mol Genet       Date:  2008-08-26       Impact factor: 6.150

6.  Metformin retards aging in C. elegans by altering microbial folate and methionine metabolism.

Authors:  Filipe Cabreiro; Catherine Au; Kit-Yi Leung; Nuria Vergara-Irigaray; Helena M Cochemé; Tahereh Noori; David Weinkove; Eugene Schuster; Nicholas D E Greene; David Gems
Journal:  Cell       Date:  2013-03-28       Impact factor: 41.582

7.  Partitioning of One-Carbon Units in Folate and Methionine Metabolism Is Essential for Neural Tube Closure.

Authors:  Kit-Yi Leung; Yun Jin Pai; Qiuying Chen; Chloe Santos; Enrica Calvani; Sonia Sudiwala; Dawn Savery; Markus Ralser; Steven S Gross; Andrew J Copp; Nicholas D E Greene
Journal:  Cell Rep       Date:  2017-11-14       Impact factor: 9.423

8.  Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues.

Authors:  Jakub Krijt; Alena Dutá; Viktor Kozich
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2009-05-28       Impact factor: 3.205

9.  Selection and Characteristics of Fermented Salted Seafood (jeotgal)-Originated Strains with Excellent S-adenosyl-L-methionine (SAM) Production and Probiotics Efficacy.

Authors:  Min-Jeong Kim; Sunhyun Park; Ran-Sook Lee; Sang-Dong Lim; Hyo Jin Kim; Myung-Ki Lee
Journal:  Korean J Food Sci Anim Resour       Date:  2014-02-28       Impact factor: 2.622

10.  Folate metabolite profiling of different cell types and embryos suggests variation in folate one-carbon metabolism, including developmental changes in human embryonic brain.

Authors:  Kit-Yi Leung; Sandra C P De Castro; Filipe Cabreiro; Peter Gustavsson; Andrew J Copp; Nicholas D E Greene
Journal:  Mol Cell Biochem       Date:  2013-03-13       Impact factor: 3.396
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