Literature DB >> 1688711

Bleomycin-treated DNA is specifically cleaved only by endonuclease IV in E. coli.

M E Hagensee1, R E Moses.   

Abstract

We have isolated an endonuclease from E. coli active on bleomycin-treated DNA. Purification on DEAE-cellulose separated this activity in strains lacking endonuclease I, endonuclease III or exonuclease III. After DEAE chromatography, the enzyme was active in the absence of divalent cations and was not inhibited by tRNA or harmane. In addition, this enzyme was stable at 45 degrees C for 20 min. These properties are consistent with this activity being endonuclease IV. This was supported by our finding no activity in a strain lacking endonuclease IV.

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Year:  1990        PMID： 1688711     DOI： 10.1016/0167-4781(90)90016-u

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  4 in total

1.  Human HeLa cell enzymes that remove phosphoglycolate 3'-end groups from DNA.

Authors:  T A Winters; M Weinfeld; T J Jorgensen
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

2.  Isolation of cDNA clones encoding a human apurinic/apyrimidinic endonuclease that corrects DNA repair and mutagenesis defects in E. coli xth (exonuclease III) mutants.

Authors:  C N Robson; I D Hickson
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

3.  Isolation of cDNA clones encoding an enzyme from bovine cells that repairs oxidative DNA damage in vitro: homology with bacterial repair enzymes.

Authors:  C N Robson; A M Milne; D J Pappin; I D Hickson
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

4.  In vitro detection of endonuclease IV-specific DNA damage formed by bleomycin in vivo.

Authors:  J D Levin; B Demple
Journal:  Nucleic Acids Res       Date:  1996-03-01       Impact factor: 16.971
  4 in total

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