BACKGROUND: The process of ingrowth of new blood vessels is stimulated by the action of vascular endothelial growth factor (VEGF), while it may be simultaneously related to the degree of tissue hypoxia. Hypoxia-inducible factor 1alpha (HIF-1alpha) is a protein of cellular response to hypoxia. The relationship between hypoxia and angiogenesis in patients with benign prostate hyperplasia (BPH) and prostate cancer (PCa) was examined. MATERIALS AND METHODS: One hundred and seventy (170) prostatic tissue samples were immunohistochemically evaluated. The microvessel density (MVD) was calculated by CD34 immunostaining; the angiogenetic profile was further assessed using a monoclonal antibody against VEGF. HIF-1alpha immunoreaction was recognized through nuclear staining of positive cells. RESULTS: CD34, VEGF and HIF-1alpha staining reactions were significantly higher in the PCa group than in the BPH group. In both groups, an interrelationship between the immunoexpression of CD34 and HIF-1alpha, VEGF and HIF-1alpha, as well as VEGF and CD34 was detected. CONCLUSION: MVD, VEGF cytoplasmic immunoreactivity and HIF-1alpha immunoreaction were more prominent in PCa than in BPH and were also significantly associated with high-grade carcinomas.
BACKGROUND: The process of ingrowth of new blood vessels is stimulated by the action of vascular endothelial growth factor (VEGF), while it may be simultaneously related to the degree of tissue hypoxia. Hypoxia-inducible factor 1alpha (HIF-1alpha) is a protein of cellular response to hypoxia. The relationship between hypoxia and angiogenesis in patients with benign prostate hyperplasia (BPH) and prostate cancer (PCa) was examined. MATERIALS AND METHODS: One hundred and seventy (170) prostatic tissue samples were immunohistochemically evaluated. The microvessel density (MVD) was calculated by CD34 immunostaining; the angiogenetic profile was further assessed using a monoclonal antibody against VEGF. HIF-1alpha immunoreaction was recognized through nuclear staining of positive cells. RESULTS:CD34, VEGF and HIF-1alpha staining reactions were significantly higher in the PCa group than in the BPH group. In both groups, an interrelationship between the immunoexpression of CD34 and HIF-1alpha, VEGF and HIF-1alpha, as well as VEGF and CD34 was detected. CONCLUSION: MVD, VEGF cytoplasmic immunoreactivity and HIF-1alpha immunoreaction were more prominent in PCa than in BPH and were also significantly associated with high-grade carcinomas.
Authors: Erin L Van Blarigan; John P Gerstenberger; June M Chan; Lorelei A Mucci; Stacey A Kenfield; Edward L Giovannucci; Meir J Stampfer; Lee W Jones; Steven K Clinton Journal: Cancer Prev Res (Phila) Date: 2015-08-14
Authors: Laura E Pascal; Junkui Ai; Lora H Rigatti; Anne K Lipton; Wuhan Xiao; James R Gnarra; Zhou Wang Journal: Angiogenesis Date: 2011-06-03 Impact factor: 9.596
Authors: M I Koukourakis; A Giatromanolaki; M Panteliadou; S E Pouliliou; P S Chondrou; S Mavropoulou; E Sivridis Journal: Br J Cancer Date: 2014-04-08 Impact factor: 7.640
Authors: Weranja K B Ranasinghe; Lin Xiao; Suzana Kovac; Mike Chang; Carine Michiels; Damien Bolton; Arthur Shulkes; Graham S Baldwin; Oneel Patel Journal: PLoS One Date: 2013-01-16 Impact factor: 3.240