Literature DB >> 16885321

Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.

Maximilian Schlicht1, Christian Berens, Janko Daam, Wolfgang Hillen.   

Abstract

The insertion element InsTipalpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.

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Year:  2006        PMID： 16885321      PMCID： PMC1538708          DOI： 10.1128/AEM.00739-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  34 in total

1.  Transposome insertional mutagenesis and direct sequencing of microbial genomes.

Authors:  L M Hoffman; J J Jendrisak; R J Meis; I Y Goryshin; S W Reznikof
Journal:  Genetica       Date:  2000       Impact factor: 1.082

2.  RNA expression analysis using a 30 base pair resolution Escherichia coli genome array.

Authors:  D W Selinger; K J Cheung; R Mei; E M Johansson; C S Richmond; F R Blattner; D J Lockhart; G M Church
Journal:  Nat Biotechnol       Date:  2000-12       Impact factor: 54.908

Review 3.  Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains.

Authors:  Thierry Rabilloud
Journal:  Proteomics       Date:  2002-01       Impact factor: 3.984

4.  Gene expression response to misfolded protein as a screen for soluble recombinant protein.

Authors:  Scott A Lesley; Jim Graziano; Charles Y Cho; Mark W Knuth; Heath E Klock
Journal:  Protein Eng       Date:  2002-02

Review 5.  Advances in proteome analysis by mass spectrometry.

Authors:  T J Griffin; D R Goodlett; R Aebersold
Journal:  Curr Opin Biotechnol       Date:  2001-12       Impact factor: 9.740

6.  The baeSR two-component regulatory system activates transcription of the yegMNOB (mdtABCD) transporter gene cluster in Escherichia coli and increases its resistance to novobiocin and deoxycholate.

Authors:  Natalya Baranova; Hiroshi Nikaido
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

7.  Large-scale analysis of the yeast genome by transposon tagging and gene disruption.

Authors:  P Ross-Macdonald; P S Coelho; T Roemer; S Agarwal; A Kumar; R Jansen; K H Cheung; A Sheehan; D Symoniatis; L Umansky; M Heidtman; F K Nelson; H Iwasaki; K Hager; M Gerstein; P Miller; G S Roeder; M Snyder
Journal:  Nature       Date:  1999-11-25       Impact factor: 49.962

8.  Epitope tagging of chromosomal genes in Salmonella.

Authors:  S Uzzau; N Figueroa-Bossi; S Rubino; L Bossi
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-11       Impact factor: 11.205

9.  Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase.

Authors:  Martin Köstner; Britta Schmidt; Ralph Bertram; Wolfgang Hillen
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

10.  Introduction of a carboxyl group in the first transmembrane helix of Escherichia coli F1Fo ATPase subunit c and cytoplasmic pH regulation.

Authors:  P C Jones
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490
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  6 in total

Review 1.  The application of Tet repressor in prokaryotic gene regulation and expression.

Authors:  Ralph Bertram; Wolfgang Hillen
Journal:  Microb Biotechnol       Date:  2008-01       Impact factor: 5.813

2.  Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence.

Authors:  Tina Gauger; Felix Weihs; Sonja Mayer; Bernhard Krismer; Jan Liese; Melanie Kull; Ralph Bertram
Journal:  Microb Biotechnol       Date:  2011-09-29       Impact factor: 5.813

Review 3.  Status quo of tet regulation in bacteria.

Authors:  Ralph Bertram; Bernd Neumann; Christopher F Schuster
Journal:  Microb Biotechnol       Date:  2021-10-29       Impact factor: 5.813

4.  Promoter strength driving TetR determines the regulatory properties of Tet-controlled expression systems.

Authors:  Christiane Georgi; Julia Buerger; Wolfgang Hillen; Christian Berens
Journal:  PLoS One       Date:  2012-07-27       Impact factor: 3.240

5.  Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site.

Authors:  Marcus Klotzsche; Dagmar Goeke; Christian Berens; Wolfgang Hillen
Journal:  Nucleic Acids Res       Date:  2007-06-01       Impact factor: 16.971

6.  Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control.

Authors:  Adam C Fisher; Matthew P DeLisa
Journal:  PLoS One       Date:  2008-06-11       Impact factor: 3.240
  6 in total

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