BACKGROUND: Isolation of enterovirus in cell culture is still utilized by clinical laboratories, for vaccine research, and for identifying serotypes for disease surveillance. Use of a combination of cell lines is recommended yet this practice has not been rigorously examined for shell vials. OBJECTIVES: To evaluate the growth predilection of enterovirus serotypes for certain shell vial cell lines in clinical samples received at a national reference laboratory. STUDY DESIGN: We retrospectively reviewed results of samples submitted for viral culture over a 3-year period. PMK, BGM, RD, A549, and MRC-5 cell lines grown in shell vials were inoculated and serotyped. RESULTS: Of 55,816 cultures, 1047 (1.9%) yielded enterovirus representing 18 serotypes. Echovirus 7, echovirus 9, and echovirus 30 were the most common serotypes recovered in 2002, 2003, and 2004, respectively. PMK and MRC-5 recovered the majority of enterovirus isolates; the addition of BGM and RD cells increased our recovery rate by 13%. For 52.6% of enteroviral isolates, cytopathic effect was found in only a single cell line. PMK and BGM cells were effective in isolating coxsackieviruses, and RD and MRC-5 were useful particularly in isolating echoviruses. CONCLUSIONS: A combination of shell vial cell lines is still recommended for recovery of enteroviruses.
BACKGROUND: Isolation of enterovirus in cell culture is still utilized by clinical laboratories, for vaccine research, and for identifying serotypes for disease surveillance. Use of a combination of cell lines is recommended yet this practice has not been rigorously examined for shell vials. OBJECTIVES: To evaluate the growth predilection of enterovirus serotypes for certain shell vial cell lines in clinical samples received at a national reference laboratory. STUDY DESIGN: We retrospectively reviewed results of samples submitted for viral culture over a 3-year period. PMK, BGM, RD, A549, and MRC-5 cell lines grown in shell vials were inoculated and serotyped. RESULTS: Of 55,816 cultures, 1047 (1.9%) yielded enterovirus representing 18 serotypes. Echovirus 7, echovirus 9, and echovirus 30 were the most common serotypes recovered in 2002, 2003, and 2004, respectively. PMK and MRC-5 recovered the majority of enterovirus isolates; the addition of BGM and RD cells increased our recovery rate by 13%. For 52.6% of enteroviral isolates, cytopathic effect was found in only a single cell line. PMK and BGM cells were effective in isolating coxsackieviruses, and RD and MRC-5 were useful particularly in isolating echoviruses. CONCLUSIONS: A combination of shell vial cell lines is still recommended for recovery of enteroviruses.
Authors: Jose L Huaman; Gladys Carrion; Julia S Ampuero; Jorge Gomez; Victor Ocaña; Irmia Paz; Elizabeth Gomez; Edward Chavez; Favio Sarmiento; Edward Pozo; V Alberto Laguna-Torres; Eric S Halsey Journal: Virol J Date: 2014-09-22 Impact factor: 4.099
Authors: Rosemary C She; Sergey N Preobrazhensky; Edward W Taggart; Cathy A Petti; David W Bahler Journal: J Virol Methods Date: 2009-09-04 Impact factor: 2.014