| Literature DB >> 16884921 |
Layla Fernández1, Ignacio Pérez-Victoria, Alberto Zafra, Pedro L Benítez, Juan C Morales, Javier Velasco, José L Adrio.
Abstract
The mature lipI gene, encoding the lipase I from Galactomyces geotrichum BT107, was obtained by PCR from genomic DNA, sequenced and cloned into a Pichia pastoris expression vector. Clones containing multiple copies of lipI integrated in their genome were analyzed to achieve high-level expression of the recombinant lipase I. One strain with four or more copies of the expression cassette was able to produce more than 200mg/L of extracellular heterologous protein. The lipase I was partially purified using anion exchange chromatography and its activity on monounsaturated (triolein) and polyunsaturated (triEPA) triglycerides was analyzed by a novel HPLC-MS assay.Entities:
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Year: 2006 PMID： 16884921 DOI： 10.1016/j.pep.2006.06.015
Source DB: PubMed Journal: Protein Expr Purif ISSN： 1046-5928 Impact factor: 1.650