| Literature DB >> 16884902 |
Yanxia Hou1, Nicole Jaffrezic-Renault, Claude Martelet, Aidong Zhang, Jasmina Minic-Vidic, Tatiana Gorojankina, Marie-Annick Persuy, Edith Pajot-Augy, Roland Salesse, Vladimir Akimov, Lino Reggiani, Cecilia Pennetta, Eleonora Alfinito, Oscar Ruiz, Gabriel Gomila, Josep Samitier, Abdelhamid Errachid.
Abstract
In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor I7 (OR I7) in its membrane fraction. The OR I7 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls.Entities:
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Year: 2006 PMID： 16884902 DOI： 10.1016/j.bios.2006.06.018
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618