Literature DB >> 16884717

Saccharomyces cerevisiae telomerase subunit Est3p binds DNA and RNA and stimulates unwinding of RNA/DNA heteroduplexes.

Yu S Sharanov1, M I Zvereva, O A Dontsova.   

Abstract

Telomerase is a key participant of telomere length maintenance system in majority of eukaryotes. It synthesizes telomere repeats at 3'-end of telomere DNA according to its own RNA template. In addition to the reverse transcriptase subunit Est2p and telomerase RNA TLC1, yeast telomerase contain Est1p, necessary for telomerase attachment to telomere and telomerase activation, and Est3p, a subunit with unknown function. We have isolated Est3p and examined its biochemical properties. Est3p binds both DNA and RNA oligonucleotides containing telomere repeat sequences and stimulates dissociation of RNA/DNA heteroduplexes. The importance of these properties of Est3p for telomerase function is discussed.

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Year:  2006        PMID： 16884717     DOI： 10.1016/j.febslet.2006.07.048

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  5 in total

1.  In vitro dimerization of telomerase protein Est3p is stimulated by magnesium cations.

Authors:  A G Malyavko; N A Logvina; M E Zvereva; O A Dontsova
Journal:  Dokl Biochem Biophys       Date:  2010-08-17       Impact factor: 0.788

2.  Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2.

Authors:  Jennell M Talley; Diane C DeZwaan; Leslie D Maness; Brian C Freeman; Katherine L Friedman
Journal:  J Biol Chem       Date:  2011-06-09       Impact factor: 5.157

3.  Telomerase regulatory subunit Est3 in two Candida species physically interacts with the TEN domain of TERT and telomeric DNA.

Authors:  Wei-Feng Yen; Lidia Chico; Ming Lei; Neal F Lue
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-17       Impact factor: 11.205

4.  The 5' arm of Kluyveromyces lactis telomerase RNA is critical for telomerase function.

Authors:  Majdi M Kabaha; Benny Zhitomirsky; Irit Schwartz; Yehuda Tzfati
Journal:  Mol Cell Biol       Date:  2008-01-14       Impact factor: 4.272

5.  Investigating the role of the Est3 protein in yeast telomere replication.

Authors:  Jaesung Lee; Edward K Mandell; Timsi Rao; Deborah S Wuttke; Victoria Lundblad
Journal:  Nucleic Acids Res       Date:  2010-01-04       Impact factor: 16.971
  5 in total

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