Literature DB >> 16884024

Nourseothricin acetyltransferease: a positive selectable marker for Toxoplasma gondii.

T Van Tam1, Peggy J Rooney, Laura J Knoll.   

Abstract

Molecular analysis of parasite genomes will require new molecular genetic tools. The nat1 gene of Streptomyces noursei encodes nourseothricin acetyltransferase, conferring resistance to the aminoglycoside antibiotic nourseothricin. Electroporation of nat1 cassettes into RH or Prugniaud strains of Toxoplasma gondii allows for selection of stable nourseothricin-resistant clones.

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Year:  2006        PMID： 16884024     DOI： 10.1645/GE-706R.1

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  3 in total

1.  Cytosolic selection systems to study protein stability.

Authors:  Ajamaluddin Malik; Antje Mueller-Schickert; James C A Bardwell
Journal:  J Bacteriol       Date:  2014-09-29       Impact factor: 3.490

2.  Functional Characterization of the Thrombospondin-Related Paralogous Proteins Rhoptry Discharge Factors 1 and 2 Unveils Phenotypic Plasticity in Toxoplasma gondii Rhoptry Exocytosis.

Authors:  Alessia Possenti; Manlio Di Cristina; Chiara Nicastro; Matteo Lunghi; Valeria Messina; Federica Piro; Lorenzo Tramontana; Simona Cherchi; Mario Falchi; Lucia Bertuccini; Furio Spano
Journal:  Front Microbiol       Date:  2022-06-09       Impact factor: 6.064

3.  Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells.

Authors:  Bose S Kochupurakkal; J Dirk Iglehart
Journal:  PLoS One       Date:  2013-07-04       Impact factor: 3.240
  3 in total

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