Literature DB >> 16880548

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of Rv2827c from Mycobacterium tuberculosis.

Robert Janowski1, Ali Nasser Eddine, Stefan H E Kaufmann, Manfred S Weiss.   

Abstract

The hypothetical protein Rv2827c from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli. It was purified using affinity and size-exclusion chromatographic techniques and then crystallized. Preliminary X-ray diffraction data analysis suggests the presence of two translationally related molecules in the asymmetric unit of the orthorhombic crystals.

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Year:  2006        PMID： 16880548      PMCID： PMC2242928          DOI： 10.1107/S1744309106024213

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  16 in total

1.  The Protein Data Bank.

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2.  SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.

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Review 3.  Structural bioinformatic approaches to the discovery of new antimycobacterial drugs.

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5.  Solvent content of protein crystals.

Authors:  B W Matthews
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6.  Unique transcriptome signature of Mycobacterium tuberculosis in pulmonary tuberculosis.

Authors:  Helmy Rachman; Michael Strong; Timo Ulrichs; Leander Grode; Johannes Schuchhardt; Hans Mollenkopf; George A Kosmiadi; David Eisenberg; Stefan H E Kaufmann
Journal:  Infect Immun       Date:  2006-02       Impact factor: 3.441

7.  The COG database: new developments in phylogenetic classification of proteins from complete genomes.

Authors:  R L Tatusov; D A Natale; I V Garkavtsev; T A Tatusova; U T Shankavaram; B S Rao; B Kiryutin; M Y Galperin; N D Fedorova; E V Koonin
Journal:  Nucleic Acids Res       Date:  2001-01-01       Impact factor: 16.971

8.  Mycobacterium tuberculosis gene expression profiling within the context of protein networks.

Authors:  Helmy Rachman; Michael Strong; Ulrich Schaible; Johannes Schuchhardt; Kristine Hagens; Hans Mollenkopf; David Eisenberg; Stefan H E Kaufmann
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9.  Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv.

Authors:  Jean-Christophe Camus; Melinda J Pryor; Claudine Médigue; Stewart T Cole
Journal:  Microbiology       Date:  2002-10       Impact factor: 2.777

10.  Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.

Authors:  S T Cole; R Brosch; J Parkhill; T Garnier; C Churcher; D Harris; S V Gordon; K Eiglmeier; S Gas; C E Barry; F Tekaia; K Badcock; D Basham; D Brown; T Chillingworth; R Connor; R Davies; K Devlin; T Feltwell; S Gentles; N Hamlin; S Holroyd; T Hornsby; K Jagels; A Krogh; J McLean; S Moule; L Murphy; K Oliver; J Osborne; M A Quail; M A Rajandream; J Rogers; S Rutter; K Seeger; J Skelton; R Squares; S Squares; J E Sulston; K Taylor; S Whitehead; B G Barrell
Journal:  Nature       Date:  1998-06-11       Impact factor: 49.962
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  1 in total

1.  Structural analysis reveals DNA binding properties of Rv2827c, a hypothetical protein from Mycobacterium tuberculosis.

Authors:  Robert Janowski; Santosh Panjikar; Ali Nasser Eddine; Stefan H E Kaufmann; Manfred S Weiss
Journal:  J Struct Funct Genomics       Date:  2009-01-31
  1 in total

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