Literature DB >> 1687561

Partial purification and properties of (Na+ + K+)-ATPase from Potamon potamios.

I Tentes1, E Stratakis.   

Abstract

1. The tissue distribution of the (Na+ + K+)-ATPase in the freshwater/land crab Potamon Potamios was studied. 2. Gills were found to display the highest total activity in the whole animal (47%) but the highest specific activity was detected in the heart (15.15 mumol Pi/mg protein/min). 3. All other organs tested were found to have low enzyme activity. 4. The freshwater/land crab ATPase enzyme was inhibited by ouabain with a Ki of 0.5 mM.Km values for ATP, Mg2+ and K+ were 1.4, 4.0 and 1.2 mM respectively. The enzyme also showed a break in the Arrhenius plot at 23 degrees C. 5. A purification method of microsomal ATPase is described involving ultracentrifugation and electrofocusing.

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Year:  1991        PMID： 1687561     DOI： 10.1016/0742-8413(91)90050-4

Source DB:  PubMed          Journal:  Comp Biochem Physiol C        ISSN： 0742-8413


  2 in total

1.  A Kinetic Characterization of the Gill (Na+, K+)-ATPase from the Semi-terrestrial Mangrove Crab Cardisoma guanhumi Latreille, 1825 (Decapoda, Brachyura).

Authors:  Daniel L Farias; Malson N Lucena; Daniela P Garçon; Fernando L Mantelatto; John C McNamara; Francisco A Leone
Journal:  J Membr Biol       Date:  2017-08-24       Impact factor: 1.843

2.  Kinetic analysis of gill (Na⁺,K⁺)-ATPase activity in selected ontogenetic stages of the Amazon River shrimp, Macrobrachium amazonicum (Decapoda, Palaemonidae): interactions at ATP- and cation-binding sites.

Authors:  Francisco Assis Leone; Douglas Chodi Masui; Thais Milena de Souza Bezerra; Daniela Pereira Garçon; Wagner Cotroni Valenti; Alessandra Silva Augusto; John Campbell McNamara
Journal:  J Membr Biol       Date:  2012-04-28       Impact factor: 1.843
  2 in total

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