BACKGROUND: Human herpesviruses cause clinically important diseases, e.g. infections of the central nervous system. New diagnostic tools are required for rapid and reliable detection of these viruses. OBJECTIVES: A microarray-based method was designed for detection of eight human herpesviruses in cerebrospinal fluid (CSF), whole blood, plasma, serum and proficiency-testing specimens. STUDY DESIGN: Herpes simplex type 1 and 2, varicella-zoster, cytomegalo-, Epstein-Barr and human herpes viruses 6A, 6B and 7 were amplified from clinical specimens by two multiplex-PCRs and transcribed to single-stranded RNAs which were hybridized to oligonucleotides on microarray. The results were compared to those from conventional PCR. In total, 227 specimens were tested including 23 CSF, 10 whole blood, 73 plasma, 10 proficiency-testing samples and 111 negative control samples. RESULTS: Concordant results were obtained in 214/227 (94%). Microarray detected 10 possible double and one triple infection. Negative control samples (70 serum, 30 CSF and 11 proficiency-testing samples) were all negative. CONCLUSIONS: Microarray is suitable for detection of multiple herpesviruses in clinical samples.
BACKGROUND:Human herpesviruses cause clinically important diseases, e.g. infections of the central nervous system. New diagnostic tools are required for rapid and reliable detection of these viruses. OBJECTIVES: A microarray-based method was designed for detection of eight human herpesviruses in cerebrospinal fluid (CSF), whole blood, plasma, serum and proficiency-testing specimens. STUDY DESIGN: Herpes simplex type 1 and 2, varicella-zoster, cytomegalo-, Epstein-Barr and human herpes viruses 6A, 6B and 7 were amplified from clinical specimens by two multiplex-PCRs and transcribed to single-stranded RNAs which were hybridized to oligonucleotides on microarray. The results were compared to those from conventional PCR. In total, 227 specimens were tested including 23 CSF, 10 whole blood, 73 plasma, 10 proficiency-testing samples and 111 negative control samples. RESULTS: Concordant results were obtained in 214/227 (94%). Microarray detected 10 possible double and one triple infection. Negative control samples (70 serum, 30 CSF and 11 proficiency-testing samples) were all negative. CONCLUSIONS: Microarray is suitable for detection of multiple herpesviruses in clinical samples.
Authors: Miguel A Martínez; María de Los Dolores Soto-Del Río; Rosa María Gutiérrez; Charles Y Chiu; Alexander L Greninger; Juan Francisco Contreras; Susana López; Carlos F Arias; Pavel Isa Journal: J Clin Microbiol Date: 2014-10-29 Impact factor: 5.948
Authors: Anne J Jääskeläinen; Sari M Viitala; Satu Kurkela; Satu Hepojoki; Heidi Sillanpää; Hannimari Kallio-Kokko; Tomas Bergström; Jukka Suni; Ale Närvänen; Olli Vapalahti; Antti Vaheri Journal: J Microbiol Methods Date: 2014-03-01 Impact factor: 2.363
Authors: Hannamari Välimaa; Maria F Perdomo; Lari Pyöriä; Maija Jokinen; Mari Toppinen; Henri Salminen; Tytti Vuorinen; Veijo Hukkanen; Constanze Schmotz; Endrit Elbasani; Päivi M Ojala; Klaus Hedman Journal: mSphere Date: 2020-06-24 Impact factor: 4.389
Authors: J O Virtanen; M Färkkilä; J Multanen; L Uotila; A J Jääskeläinen; A Vaheri; M Koskiniemi Journal: J Neurovirol Date: 2007-08 Impact factor: 3.739