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Literature DB >> 16871026

Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf).

Geewook Shin¹, Hyungjun Lee, K J Palaksha, Youngrim Kim, Eunyoung Lee, Yongseung Shin, Eunggoo Lee, Kyungdae Park, Taesung Jung.

Abstract

The present study was undertaken to produce monoclonal antibodies (MAbs) against immunoglobulin (Ig) purified from black rockfish (Sebastes schlegeli Higendorf) serum using protein A, mannan binding protein, and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only 0.46 M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE; they also had similar antigenicity for MAbs to Ig heavy chain in immunoblot assays. Therefore, black rockfish Ig is believed to exist as a single isotype within serum. The MAbs produced against Ig heavy chain reacted specifically with spots distributed over the pI range from 4.8 to 5.6 with a molecular weight of 70 kDa on two dimensional gel electrophoresis immunoblot profiles.

Entities: CellLine Chemical Disease Gene Species

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Year: 2006 PMID： 16871026 PMCID： PMC3242131 DOI： 10.4142/jvs.2006.7.3.293

Source DB: PubMed Journal: J Vet Sci ISSN： 1229-845X Impact factor: 1.672

11 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity. 1975.

Authors: G Köhler; C Milstein
Journal: J Immunol Date: 2005-03-01 Impact factor: 5.422

2. Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli).

Authors: Sung Hyun Kang; Gee Wook Shin; Yong Seung Shin; Palaksha K J; Young-rim Kim; Hyang-hee Yang; Eun-young Lee; Eung-goo Lee; Nam-eung Huh; Oh Myung Ju; Tae-sung Jung
Journal: J Vet Sci Date: 2004-12 Impact factor: 1.672

3. Phylogeny of immunoglobulin structure and function. IV. Immunoglobulins of the giant grouper, Epinephelus itaira.

Authors: L W Clem
Journal: J Biol Chem Date: 1971-01-10 Impact factor: 5.157

4. Two-dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM-associated peptide.

Authors: J D Tissot; J A Schifferli; D F Hochstrasser; C Pasquali; F Spertini; F Clément; S Frutiger; N Paquet; G J Hughes; P Schneider
Journal: J Immunol Methods Date: 1994-07-12 Impact factor: 2.303

5. Evolution of antibody structure and effector functions: comparative hemolytic activities of monomeric and tetrameric IgM from rainbow trout, Salmo gairdnerii.

Authors: B M Elcombe; R J Chang; C J Taves; J L Winkelhake
Journal: Comp Biochem Physiol B Date: 1985

Review 2. Potential Applications of Fluorescence-Activated Cell Sorting (FACS) and Droplet-Based Microfluidics in Promoting the Discovery of Specific Antibodies for Characterizations of Fish Immune Cells.

Authors: Chenjie Fei; Li Nie; Jianhua Zhang; Jiong Chen
Journal: Front Immunol Date: 2021-11-16 Impact factor: 7.561

2 in total

Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf).

1. Continuous cultures of fused cells secreting antibody of predefined specificity. 1975.

2. Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli).

3. Phylogeny of immunoglobulin structure and function. IV. Immunoglobulins of the giant grouper, Epinephelus itaira.

4. Two-dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM-associated peptide.

5. Evolution of antibody structure and effector functions: comparative hemolytic activities of monomeric and tetrameric IgM from rainbow trout, Salmo gairdnerii.

6. Discovery of a new class of immunoglobulin heavy chain from fugu.

7. Analysis of two IgM isotypes in Atlantic salmon and brown trout.

8. Differences in mucus and serum immunoglobulin of carp (Cyprinus carpio L.).

9. Immunoglobulin heavy H chain isotypes in a teleost fish.

10. Characterization of serum immunoglobulin M of the Antarctic teleost Trematomus bernacchii.

1. Complete genome sequence and immunoproteomic analyses of the bacterial fish pathogen Streptococcus parauberis.

Review 2. Potential Applications of Fluorescence-Activated Cell Sorting (FACS) and Droplet-Based Microfluidics in Promoting the Discovery of Specific Antibodies for Characterizations of Fish Immune Cells.