Literature DB >> 1687029

Evaluation of fingerprinting methods for identification of Helicobacter pylori strains.

C L Clayton1, H Kleanthous, J C Dent, C A McNulty, S Tabaqchali.   

Abstract

Variation amongst strains of Helicobacter pylori was examined by 35S-methionine-labelled protein SDS-PAGE (Radio-PAGE), immunoblot and DNA fingerprinting techniques. These methods allowed discrimination amongst isolates and showed total correlation. Pig and baboon gastric Helicobacter pylori-like organisms were found to be very similar to Helicobacter pylori by both Radio-PAGE and immunoblotting, whereas a Helicobacter mustelae isolate was markedly different. The HindIII restriction endonuclease digest patterns of Helicobacter pylori DNA illustrated the considerable genomic variation of this organism. The Radio-PAGE and immunoblot typing methods both gave precise identification of Helicobacter pylori strains, but Radio-PAGE was found to give higher resolution and represents a standardised universally applicable fingerprinting method for Helicobacter pylori.

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Year:  1991        PMID： 1687029     DOI： 10.1007/bf01984926

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  35 in total

1.  Evidence of gastritis with several Helicobacter pylori strains.

Authors:  A Beji; P Vincent; I Darchis; M O Husson; A Cortot; H Leclerc
Journal:  Lancet       Date:  1989-12-09       Impact factor: 79.321

2.  Campylobacter pyloridis and associated gastritis: investigator blind, placebo controlled trial of bismuth salicylate and erythromycin ethylsuccinate.

Authors:  C A McNulty; J C Gearty; B Crump; M Davis; I A Donovan; V Melikian; D M Lister; R Wise
Journal:  Br Med J (Clin Res Ed)       Date:  1986-09-13

3.  Campylobacter pyloridis-associated chronic active antral gastritis. A prospective study of its prevalence and the effects of antibacterial and antiulcer treatment.

Authors:  E A Rauws; W Langenberg; H J Houthoff; H C Zanen; G N Tytgat
Journal:  Gastroenterology       Date:  1988-01       Impact factor: 22.682

4.  Immunoblot fingerprinting of Campylobacter pylori.

Authors:  J P Burnie; W Lee; J C Dent; C A McNulty
Journal:  J Med Microbiol       Date:  1988-10       Impact factor: 2.472

5.  New method for typing coagulase negative staphylococci.

Authors:  J R Stephenson; S Tabaqchali
Journal:  J Clin Pathol       Date:  1986-11       Impact factor: 3.411

6.  Signature patterns of DNA restriction fragments of Helicobacter pylori before and after treatment.

Authors:  R J Owen; J Fraser; M Costas; D Morgan; D R Morgan
Journal:  J Clin Pathol       Date:  1990-08       Impact factor: 3.411

7.  Isolation of a gastric campylobacter-like organism from the stomach of four rhesus monkeys, and identification as Campylobacter pylori.

Authors:  D G Newell; M J Hudson; A Baskerville
Journal:  J Med Microbiol       Date:  1988-09       Impact factor: 2.472

8.  Strain variation in Campylobacter pylori detected by numerical analysis of one-dimensional electrophoretic protein patterns.

Authors:  R J Owen; M Costas; D D Morgan; S L On; L R Hill; A D Pearson; D R Morgan
Journal:  Antonie Van Leeuwenhoek       Date:  1989-03       Impact factor: 2.271

9.  Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis.

Authors:  C K Kakoyiannis; P J Winter; R B Marshall
Journal:  Appl Environ Microbiol       Date:  1984-09       Impact factor: 4.792

Review 10.  Campylobacter pylori and peptic ulcer disease.

Authors:  D Y Graham
Journal:  Gastroenterology       Date:  1989-02       Impact factor: 22.682
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  6 in total

1.  Adaptation of Helicobacter pylori to aerobic growth.

Authors:  D S Tompkins; J Dave; N P Mapstone
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-05       Impact factor: 3.267

2.  Performance criteria of DNA fingerprinting methods for typing of Helicobacter pylori isolates: experimental results and meta-analysis.

Authors:  C Burucoa; V Lhomme; J L Fauchere
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

3.  Differentiation of Helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay.

Authors:  S O Hynes; S Hirmo; T Wadström; A P Moran
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

4.  Differentiation of Helicobacter pylori strains directly from gastric biopsy specimens by PCR-based restriction fragment length polymorphism analysis without culture.

Authors:  C Li; T Ha; D S Chi; D A Ferguson; C Jiang; J J Laffan; E Thomas
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

5.  Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis.

Authors:  C L Clayton; H Kleanthous; D D Morgan; L Puckey; S Tabaqchali
Journal:  J Clin Microbiol       Date:  1993-06       Impact factor: 5.948

6.  Use of a receiver operating characteristic in the evaluation of two commercial enzyme immunoassays for detection of Helicobacter pylori infection.

Authors:  M Trautmann; M Moldrzyk; K Vogt; J Körber; T Held; R Marre
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-10       Impact factor: 3.267
  6 in total

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