Reversed-phase ion-pair high-performance liquid chromatography of adriamycin and adriamycinol in rat serum and tissues.

An ion-pair reversed-phase high-performance liquid chromatographic method is presented for the determination of adriamycin and adriamycinol concentrations in rat serum and tissues. The scheme for developing the chromatographic conditions is discussed. A good separation of adriamycinol and adriamycin was obtained by using a high sodium lauryl sulphate concentration in the mobile phase. The retention times of adriamycinol, adriamycin and daunomycin (internal standard) were less than 3 min with no interfering peaks from endogenous constituents. A protein precipitation method was used to prepare the serum and tissue samples for injection.