| Literature DB >> 16863986 |
Mildred M Pelletier1, Petra Kleinbongard, Lorna Ringwood, Rania Hito, Christian J Hunter, Alan N Schechter, Mark T Gladwin, André Dejam.
Abstract
There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.Entities:
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Year: 2006 PMID： 16863986 DOI： 10.1016/j.freeradbiomed.2006.05.001
Source DB: PubMed Journal: Free Radic Biol Med ISSN： 0891-5849 Impact factor: 7.376