Literature DB >> 16861753

In situ PCR protocols.

Omar Bagasra1, Twaina Harris.   

Abstract

This chapter provides detailed methods and material descriptions of in situ polymerase chain reaction protocols. It describes all the essential components of the technique, various protocols suitable for different kinds of tissues and cell preparations, and also gives various instructions for troubleshooting. We have declined to devote detailed discussions on in situ hybridization because there are several excellent articles and books available on this subject.

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Year:  2006        PMID: 16861753     DOI: 10.1385/1-59745-068-5:61

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  1 in total

1.  In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain.

Authors:  Laura Cubas-Nuñez; María Duran-Moreno; Jessica Castillo-Villalba; Jorge Fuentes-Maestre; Bonaventura Casanova; José M García-Verdugo; Sara Gil-Perotín
Journal:  Front Cell Neurosci       Date:  2017-05-16       Impact factor: 5.505

  1 in total

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