| Literature DB >> 16861024 |
Mark D G Pinches1, Christopher R Helps, Tim J Gruffydd-Jones, Kathy Egan, Oswald Jarrett, Séverine Tasker.
Abstract
In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.Entities:
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Year: 2006 PMID： 16861024 DOI： 10.1016/j.jfms.2006.05.008
Source DB: PubMed Journal: J Feline Med Surg ISSN： 1098-612X Impact factor: 2.015