BACKGROUND: In the frame of a research line dedicated to better clarify the role of exopolysaccharides (EPS) in bacterial virulence, EPS produced by species of the Burkholderia cepacia complex (Bcc), namely Burkholderia multivorans, Burkholderia cenocepacia, and a Bcc member of undetermined genomovar, all isolated at the Cystic Fibrosis Regional Centre of Florence (Italy), were investigated for they structural properties. METHODS: Three strains of B. multivorans, three of B. cenocepacia and one of a Bcc member of undetermined genomovar were isolated from CF patients. The reference strains C1576 and J2315, for genomovar II and III, respectively, were included in the study. The bacteria were grown on solid media, the exopolysaccharides produced were purified, and their structures were determined. In addition, sugar analysis of sputum samples was accomplished to search for EPS produced in vivo. RESULTS: Six strains out of seven produced the exopolysaccharide cepacian, while one strain of B. multivorans produced a completely different polymer, previously known in the literature as PS1. Two strains synthesised very small amounts of EPS. No definitive evidence for the presence of cepacian in sputum samples was found. CONCLUSIONS: Most strains examined produced abundant amounts of polysaccharides. Cepacian was the most common EPS isolated and its production was not associated to a particular genomovar.
BACKGROUND: In the frame of a research line dedicated to better clarify the role of exopolysaccharides (EPS) in bacterial virulence, EPS produced by species of the Burkholderia cepacia complex (Bcc), namely Burkholderia multivorans, Burkholderia cenocepacia, and a Bcc member of undetermined genomovar, all isolated at the Cystic Fibrosis Regional Centre of Florence (Italy), were investigated for they structural properties. METHODS: Three strains of B. multivorans, three of B. cenocepacia and one of a Bcc member of undetermined genomovar were isolated from CFpatients. The reference strains C1576 and J2315, for genomovar II and III, respectively, were included in the study. The bacteria were grown on solid media, the exopolysaccharides produced were purified, and their structures were determined. In addition, sugar analysis of sputum samples was accomplished to search for EPS produced in vivo. RESULTS: Six strains out of seven produced the exopolysaccharidecepacian, while one strain of B. multivorans produced a completely different polymer, previously known in the literature as PS1. Two strains synthesised very small amounts of EPS. No definitive evidence for the presence of cepacian in sputum samples was found. CONCLUSIONS: Most strains examined produced abundant amounts of polysaccharides. Cepacian was the most common EPS isolated and its production was not associated to a particular genomovar.
Authors: James E A Zlosnik; Trevor J Hird; Monica C Fraenkel; Leonilde M Moreira; Deborah A Henry; David P Speert Journal: J Clin Microbiol Date: 2008-02-06 Impact factor: 5.948
Authors: Ana S Ferreira; Jorge H Leitão; Inês N Silva; Pedro F Pinheiro; Sílvia A Sousa; Christian G Ramos; Leonilde M Moreira Journal: Appl Environ Microbiol Date: 2009-11-30 Impact factor: 4.792
Authors: Matthew T G Holden; Helena M B Seth-Smith; Lisa C Crossman; Mohammed Sebaihia; Stephen D Bentley; Ana M Cerdeño-Tárraga; Nicholas R Thomson; Nathalie Bason; Michael A Quail; Sarah Sharp; Inna Cherevach; Carol Churcher; Ian Goodhead; Heidi Hauser; Nancy Holroyd; Karen Mungall; Paul Scott; Danielle Walker; Brian White; Helen Rose; Pernille Iversen; Dalila Mil-Homens; Eduardo P C Rocha; Arsenio M Fialho; Adam Baldwin; Christopher Dowson; Bart G Barrell; John R Govan; Peter Vandamme; C Anthony Hart; Eshwar Mahenthiralingam; Julian Parkhill Journal: J Bacteriol Date: 2008-10-17 Impact factor: 3.490
Authors: Gabriella Pessi; Rubina Braunwalder; Alexander Grunau; Ulrich Omasits; Christian H Ahrens; Leo Eberl Journal: PLoS One Date: 2013-09-02 Impact factor: 3.240