Literature DB >> 16850559

Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference.

R M Warren1, N C Gey van Pittius, M Barnard, A Hesseling, E Engelke, M de Kock, M C Gutierrez, G K Chege, T C Victor, E G Hoal, P D van Helden.   

Abstract

Differentiation of members of the Mycobacterium tuberculosis complex by conventional mycobacteriological methods is time consuming, making surveillance of species-specific disease difficult. A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1(mic), RD2(seal), RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes.

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Year:  2006        PMID： 16850559

Source DB:  PubMed          Journal:  Int J Tuberc Lung Dis        ISSN： 1027-3719            Impact factor:   2.373


  100 in total

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2.  Early bactericidal activity and pharmacokinetics of PA-824 in smear-positive tuberculosis patients.

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Journal:  Antimicrob Agents Chemother       Date:  2010-05-24       Impact factor: 5.191

3.  Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based single nucleotide polymorphism genotyping assay using iPLEX gold technology for identification of Mycobacterium tuberculosis complex species and lineages.

Authors:  C Bouakaze; C Keyser; A Gonzalez; W Sougakoff; N Veziris; H Dabernat; B Jaulhac; B Ludes
Journal:  J Clin Microbiol       Date:  2011-07-06       Impact factor: 5.948

4.  Isolation and molecular characterization of Mycobacterium tuberculosis from humans and cattle in Namwala District, Zambia.

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5.  Disseminated Mycobacterium bovis infection in red foxes (Vulpes vulpes) with cerebral involvement found in Portugal.

Authors:  Ana C Matos; Luis Figueira; Maria H Martins; Manuela Matos; Márcia Morais; Ana P Dias; Maria L Pinto; Ana C Coelho
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6.  Randomized dose-ranging study of the 14-day early bactericidal activity of bedaquiline (TMC207) in patients with sputum microscopy smear-positive pulmonary tuberculosis.

Authors:  Andreas H Diacon; Rodney Dawson; Florian Von Groote-Bidlingmaier; Gregory Symons; Amour Venter; Peter R Donald; Almari Conradie; Ngozi Erondu; Ann M Ginsberg; Erica Egizi; Helen Winter; Piet Becker; Carl M Mendel
Journal:  Antimicrob Agents Chemother       Date:  2013-03-04       Impact factor: 5.191

7.  Mycobacterium tuberculosis and Mycobacterium africanum in stools from children attending an immunization clinic in Ibadan, Nigeria.

Authors:  S I B Cadmus; A O Jenkins; J Godfroid; K Osinusi; I F Adewole; R L Murphy; B O Taiwo
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8.  Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR.

Authors:  Chie Nakajima; Zeaur Rahim; Yukari Fukushima; Isamu Sugawara; Adri G M van der Zanden; Aki Tamaru; Yasuhiko Suzuki
Journal:  BMC Infect Dis       Date:  2010-05-15       Impact factor: 3.090

9.  Distinct genotypic profiles of the two major clades of Mycobacterium africanum.

Authors:  Sidra E Gonçalves Vasconcellos; Richard C Huard; Stefan Niemann; Kristin Kremer; Adalberto R Santos; Philip N Suffys; John L Ho
Journal:  BMC Infect Dis       Date:  2010-03-29       Impact factor: 3.090

10.  MIC of Delamanid (OPC-67683) against Mycobacterium tuberculosis Clinical Isolates and a Proposed Critical Concentration.

Authors:  Kelly Stinson; Natalia Kurepina; Amour Venter; Mamoru Fujiwara; Masanori Kawasaki; Juliano Timm; Elena Shashkina; Barry N Kreiswirth; Yongge Liu; Makoto Matsumoto; Lawrence Geiter
Journal:  Antimicrob Agents Chemother       Date:  2016-05-23       Impact factor: 5.191
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