PURPOSE: To establish proper contact lens care as a common practice by discovering the quantity and condition of protein deposition on various types of soft contact lenses. METHODS: The amounts of deposition of two proteins found in tear fluid, lysozyme and albumin, on various types of contact lenses were measured. Lenses deposited with albumin and lenses deposited with lysozyme were stained with Coomassie brilliant blue (CBB). Frozen cross-sections of these lenses were examined using a microscope. RESULTS: A considerably larger amount of lysozyme was found on group IV (U.S. Food and Drug Administration classification) contact lenses. Conversely, there were no significant differences between the four groups in the quantity of albumin found. When various lenses were stained with CBB, only the lysozyme-deposited lenses of group IV obtained a strong tint. When cross-sections of the tinted group IV contact lenses were examined, not only the surface, but also the matrix of the lens was stained. CONCLUSIONS: Results suggested that the reason group IV lenses accumulated a considerably greater amount of lysozyme than did lenses of other groups is that lysozyme accumulates not only on the surface, but also in the matrix of group IV lenses.
PURPOSE: To establish proper contact lens care as a common practice by discovering the quantity and condition of protein deposition on various types of soft contact lenses. METHODS: The amounts of deposition of two proteins found in tear fluid, lysozyme and albumin, on various types of contact lenses were measured. Lenses deposited with albumin and lenses deposited with lysozyme were stained with Coomassie brilliant blue (CBB). Frozen cross-sections of these lenses were examined using a microscope. RESULTS: A considerably larger amount of lysozyme was found on group IV (U.S. Food and Drug Administration classification) contact lenses. Conversely, there were no significant differences between the four groups in the quantity of albumin found. When various lenses were stained with CBB, only the lysozyme-deposited lenses of group IV obtained a strong tint. When cross-sections of the tinted group IV contact lenses were examined, not only the surface, but also the matrix of the lens was stained. CONCLUSIONS: Results suggested that the reason group IV lenses accumulated a considerably greater amount of lysozyme than did lenses of other groups is that lysozyme accumulates not only on the surface, but also in the matrix of group IV lenses.
Authors: Daniel R Powell; Mirunalni Thangavelu; Heather L Chandler; Kelly K Nichols; Jason J Nichols Journal: Optom Vis Sci Date: 2010-11 Impact factor: 1.973
Authors: Zhenjun Zhao; Xiaojia Wei; Yulina Aliwarga; Nicole A Carnt; Qian Garrett; Mark D P Willcox Journal: Mol Vis Date: 2008-11-07 Impact factor: 2.367