| Literature DB >> 16842581 |
H Mayta1, M Calderon, J Taverna, S Montenegro, J Balqui, K Campos, I Tuero, J Arevalo, A Vivar, R H Gilman.
Abstract
Neisseria gonorrhoeae is the most common sexually transmitted disease-causing bacterium worldwide. An in-house PCR assay targeting the carbamoyl-phosphate synthase subunit A (carA) gene was developed for the specific detection of N. gonorrhoeae in clinical specimens. Samples from 605 patients were cultured on selective medium and assayed by PCR in a double-blind fashion. Of 605 urethral/cervical samples analysed, 13 were PCR-positive, of which 11 were culture-positive. The PCR showed a sensitivity and specificity of 100% and 99.7% with these samples. PCR targeting the carA gene appears to be a reliable method for the detection of N. gonorrhoeae in clinical specimens.Entities:
Mesh：
Year: 2006 PMID： 16842581 DOI： 10.1111/j.1469-0691.2006.01452.x
Source DB: PubMed Journal: Clin Microbiol Infect ISSN： 1198-743X Impact factor: 8.067