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Literature DB >> 16840781

In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli.

Petra Born¹, Eefjan Breukink, Waldemar Vollmer.

Abstract

The penicillin-binding protein (PBP) 1A is a major murein (peptidoglycan) synthase in Escherichia coli. The murein synthesis activity of PBP1A was studied in vitro with radioactive lipid II substrate. PBP1A produced murein glycan strands by transglycosylation and formed peptide cross-links by transpeptidation. Time course experiments revealed that PBP1A, unlike PBP1B, required the presence of polymerized glycan strands carrying monomeric peptides for cross-linking activity. PBP1A was capable of attaching nascent murein synthesized from radioactive lipid II to nonlabeled murein sacculi. The attachment of the new material occurred by transpeptidation reactions in which monomeric triand tetrapeptides in the sacculi were the acceptors.

Entities: Chemical Gene Species

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Year: 2006 PMID： 16840781 DOI： 10.1074/jbc.M604083200

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

53 in total

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In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli.

1. Plasticity of Escherichia coli cell wall metabolism promotes fitness and antibiotic resistance across environmental conditions.

2. The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with penicillin-binding protein 3, FtsW, and FtsN.

3. Purification, crystallization and preliminary X-ray crystallographic analysis of Lmo0540 from Listeria monocytogenes.

4. Coarse-grained simulations of bacterial cell wall growth reveal that local coordination alone can be sufficient to maintain rod shape.

5. Analysis of genes encoding penicillin-binding proteins in clinical isolates of Acinetobacter baumannii.

6. Mechanical Genomics Identifies Diverse Modulators of Bacterial Cell Stiffness.

7. Roles for both FtsA and the FtsBLQ subcomplex in FtsN-stimulated cell constriction in Escherichia coli.

8. Interaction and modulation of two antagonistic cell wall enzymes of mycobacteria.

9. Forming cross-linked peptidoglycan from synthetic gram-negative Lipid II.

10. Tuning the moenomycin pharmacophore to enable discovery of bacterial cell wall synthesis inhibitors.