L G Buchynska1, I P Nesina. 1. R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, Kyiv, Ukraine. lubov@onconet.kiev.ua
Abstract
AIM: To study correlation links between expression level of proteins p53, p21(WAF1/CIP), p16(INK4) and proliferative potential in human endometrial adenocarcinoma (EC). MATERIAL AND METHODS: The immunohistochemical analysis of expression level of Ki-67, p53, p21(WAF1/CIP) and p16(INK4) was carried out on surgically resected endometrial cancer samples (n = 74). Scrapes of normal endometrium from 10 patients with polyps of cervical canal of the uterus served as the control. RESULTS: The data showed that endometrial malignant tumors possess high proliferative activity (proliferation index was 37.3 +/- 0.2%), overexpression of p53 (labeling index (LI) = 46.1 +/- 0.5%) and high expression of p21(WAF1/CIP) (LI = 11.2 +/- 0.4%) and p16(INK4) (LI = 12.0 +/- 0.2%). In low differentiated endometrial adenocarcinomas the highest level of Ki-67, p53 and p21(WAF1/CIP) expression and lowest content of p16(INK4) protein were observed. CONCLUSION: The indicated markers may be used along with traditional morphological and clinical characteristics for diagnosis of endometrial neoplasia.
AIM: To study correlation links between expression level of proteins p53, p21(WAF1/CIP), p16(INK4) and proliferative potential in humanendometrial adenocarcinoma (EC). MATERIAL AND METHODS: The immunohistochemical analysis of expression level of Ki-67, p53, p21(WAF1/CIP) and p16(INK4) was carried out on surgically resected endometrial cancer samples (n = 74). Scrapes of normal endometrium from 10 patients with polyps of cervical canal of the uterus served as the control. RESULTS: The data showed that endometrial malignant tumors possess high proliferative activity (proliferation index was 37.3 +/- 0.2%), overexpression of p53 (labeling index (LI) = 46.1 +/- 0.5%) and high expression of p21(WAF1/CIP) (LI = 11.2 +/- 0.4%) and p16(INK4) (LI = 12.0 +/- 0.2%). In low differentiated endometrial adenocarcinomas the highest level of Ki-67, p53 and p21(WAF1/CIP) expression and lowest content of p16(INK4) protein were observed. CONCLUSION: The indicated markers may be used along with traditional morphological and clinical characteristics for diagnosis of endometrial neoplasia.
Authors: Pradeep R Rai; Carlyne D Cool; Judy A C King; Troy Stevens; Nana Burns; Robert A Winn; Michael Kasper; Norbert F Voelkel Journal: Am J Respir Crit Care Med Date: 2008-06-12 Impact factor: 21.405