AIM: To investigate human telomerase reverse transcriptase (hTERT) mRNA in the serum of cholangiocarcinoma patients. METHODS: The serum of thirty three cholangiocarcinoma patients, forty one benign biliary tract disease patients and ten healthy volunteers were collected and analyzed for the expression of hTERT mRNA by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). We then examined the correlation between values of serum hTERT mRNA and the pathological staging of cholangiocarcinoma. RESULTS: hTERT mRNA was detected in 28 of 33 (84.85%) of serum obtained from cholangiocarcinoma patients and 9 of 41 (21.9%) of serum obtained from benign biliary tract disease patients. hTERT mRNA was not detected in any serum obtained from healthy volunteers. on the other hand the common tumor marker, CA19-9 was detected in 20 of 33 (60.6%) of serum obtained from cholangiocarcinoma patients and 8 of 41 (19.5%) of serum obtained from benign biliary tract disease patients. However, no correlation was found between the present of serum hTERT mRNA and tumor staging. CONCLUSION: These results indicate that the detection of circulating hTERT mRNA was identified in almost all cholangiocarcinoma patients. It offers a novel tumor marker, which can be used as a complementary study for diagnosis of cholangiocarcinoma.
AIM: To investigate humantelomerase reverse transcriptase (hTERT) mRNA in the serum of cholangiocarcinomapatients. METHODS: The serum of thirty three cholangiocarcinomapatients, forty one benign biliary tract diseasepatients and ten healthy volunteers were collected and analyzed for the expression of hTERT mRNA by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). We then examined the correlation between values of serum hTERT mRNA and the pathological staging of cholangiocarcinoma. RESULTS:hTERT mRNA was detected in 28 of 33 (84.85%) of serum obtained from cholangiocarcinomapatients and 9 of 41 (21.9%) of serum obtained from benign biliary tract diseasepatients. hTERT mRNA was not detected in any serum obtained from healthy volunteers. on the other hand the common tumor marker, CA19-9 was detected in 20 of 33 (60.6%) of serum obtained from cholangiocarcinomapatients and 8 of 41 (19.5%) of serum obtained from benign biliary tract diseasepatients. However, no correlation was found between the present of serum hTERT mRNA and tumor staging. CONCLUSION: These results indicate that the detection of circulating hTERT mRNA was identified in almost all cholangiocarcinomapatients. It offers a novel tumor marker, which can be used as a complementary study for diagnosis of cholangiocarcinoma.
Authors: X Q Chen; H Bonnefoi; M F Pelte; J Lyautey; C Lederrey; S Movarekhi; P Schaeffer; H E Mulcahy; P Meyer; M Stroun; P Anker Journal: Clin Cancer Res Date: 2000-10 Impact factor: 12.531
Authors: M Fleischhacker; T Beinert; M Ermitsch; D Seferi; K Possinger; C Engelmann; B Jandrig Journal: Ann N Y Acad Sci Date: 2001-09 Impact factor: 5.691