| Literature DB >> 16829126 |
Isaac Túnez1, Juan A Collado, Francisco J Medina, M Carmen Muñoz, Rafael Gordillo, Concepción Sampedro, María J Moyano, Montserrat Feijóo, Jordi Muntané, Pedro Montilla.
Abstract
The effect of carvedilol on oxidative and cell damage induced by okadaic acid in N1E-115 cells were studied. The effects of okadaic acid were evaluated as changes in: the quantity of lipid peroxidation products, protein carbonyl groups, reduced glutathione content (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase and total lactate dehydrogenase (cell LDH). Additionally, a dose of carvedilol (10(-5)M) was added 2h before incubation with okadaic acid (50 nM) and was present until the end of the experiment (2h later added okadaic acid). Our results reveal that okadaic acid induces oxidative stress and an increase of cell LDH in N1E-115 cells, whereas carvedilol prevented the changes prompted by okadaic acid. In conclusion, the data show the protective effect of carvedilol, as well as its ability to modify cell response to okadaic acid, involving like cytoprotective mechanism its antioxidative properties.Entities:
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Year: 2006 PMID： 16829126 DOI： 10.1016/j.phrs.2006.05.004
Source DB: PubMed Journal: Pharmacol Res ISSN： 1043-6618 Impact factor: 7.658