| Literature DB >> 16828249 |
Liping Wang1, Lin Lin, Baoxian Ye.
Abstract
The interaction between anticancer herbal drug emodin and DNA was studied with differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at the bare or DNA modified GCE and ultraviolet-visible (UV) spectra. The DPV of emodin showed that peak potentials shifted to more positive value and peak currents decreased with the addition of DNA. UV spectra exhibited that the absorption of emodin at about 440nm decreased with red shift. The results showed that the herbal drug emodin interacted with DNA by intercalating into the double helix of DNA. Under our experiment conditions, the decrease of peak current was proportional to DNA concentration, which can be applied to determining DNA concentration. The combining constant (beta) and combing number (m) of DNA-mEM were determined too.Entities:
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Year: 2006 PMID： 16828249 DOI： 10.1016/j.jpba.2006.05.017
Source DB: PubMed Journal: J Pharm Biomed Anal ISSN： 0731-7085 Impact factor: 3.935