BACKGROUND: The low density lipoprotein receptor (LDLR) family is a family of structurally related cell surface receptors with conserved exon/intron organization. Several members of this family have been shown to undergo alternative splicing. However, no alternative splicing of the LDLR pre-mRNA has so far been described. METHODS: In the present study alternative splicing of human LDLR pre-mRNA has been studied in eight different tissues and four different cell lines using reverse transcription (RT) PCR. A quantitative real-time PCR with exon-exon boundary spanning primers was established to measure the relative amount of two novel isoforms. RESULTS: Several novel isoforms were identified by RT-PCR of which the isoforms lacking exon 4 or 12 were two of the most prominent. Although highly detectable by RT-PCR, the quantification by real-time PCR revealed low levels of these isoforms. CONCLUSIONS: Novel isoforms of LDLR mRNA are described. Quantification by real-time PCR of two of the alternatively spliced isoforms revealed low amount of these isoforms in the examined tissues and cell lines. Further investigations are needed to evaluate if these isoforms represent functional transcripts of LDLR mRNA.
BACKGROUND: The low density lipoprotein receptor (LDLR) family is a family of structurally related cell surface receptors with conserved exon/intron organization. Several members of this family have been shown to undergo alternative splicing. However, no alternative splicing of the LDLR pre-mRNA has so far been described. METHODS: In the present study alternative splicing of humanLDLR pre-mRNA has been studied in eight different tissues and four different cell lines using reverse transcription (RT) PCR. A quantitative real-time PCR with exon-exon boundary spanning primers was established to measure the relative amount of two novel isoforms. RESULTS: Several novel isoforms were identified by RT-PCR of which the isoforms lacking exon 4 or 12 were two of the most prominent. Although highly detectable by RT-PCR, the quantification by real-time PCR revealed low levels of these isoforms. CONCLUSIONS: Novel isoforms of LDLR mRNA are described. Quantification by real-time PCR of two of the alternatively spliced isoforms revealed low amount of these isoforms in the examined tissues and cell lines. Further investigations are needed to evaluate if these isoforms represent functional transcripts of LDLR mRNA.
Authors: Sadik H Kassim; Luk H Vandenberghe; Ruben Hovhannisyan; James M Wilson; Daniel J Rader Journal: Physiol Genomics Date: 2011-05-31 Impact factor: 3.107
Authors: Marisa W Medina; Frederick Bauzon; Devesh Naidoo; Elizabeth Theusch; Kristen Stevens; Jessica Schilde; Christian Schubert; Lara M Mangravite; Lawrence L Rudel; Ryan E Temel; Heiko Runz; Ronald M Krauss Journal: Arterioscler Thromb Vasc Biol Date: 2014-07-17 Impact factor: 8.311
Authors: Haiyan Zhu; H Michael Tucker; Karrie E Grear; James F Simpson; Alisa K Manning; L Adrienne Cupples; Steven Estus Journal: Hum Mol Genet Date: 2007-05-21 Impact factor: 6.150
Authors: Marisa Wong Medina; Feng Gao; Devesh Naidoo; Lawrence L Rudel; Ryan E Temel; Allison L McDaniel; Stephanie M Marshall; Ronald M Krauss Journal: PLoS One Date: 2011-04-29 Impact factor: 3.240