| Literature DB >> 16823372 |
Akihisa Matsuyama1, Ritsuko Arai, Yoko Yashiroda, Atsuko Shirai, Ayako Kamata, Shigeko Sekido, Yumiko Kobayashi, Atsushi Hashimoto, Makiko Hamamoto, Yasushi Hiraoka, Sueharu Horinouchi, Minoru Yoshida.
Abstract
Cloning of the entire set of an organism's protein-coding open reading frames (ORFs), or 'ORFeome', is a means of connecting the genome to downstream 'omics' applications. Here we report a proteome-scale study of the fission yeast Schizosaccharomyces pombe based on cloning of the ORFeome. Taking advantage of a recombination-based cloning system, we obtained 4,910 ORFs in a form that is readily usable in various analyses. First, we evaluated ORF prediction in the fission yeast genome project by expressing each ORF tagged at the 3' terminus. Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1.Entities:
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Year: 2006 PMID： 16823372 DOI： 10.1038/nbt1222
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908