Literature DB >> 1682145

Luminography--a new, highly sensitive visualization method for electrophoresis.

R Schneppenheim1, U Budde, N Dahlmann, P Rautenberg.   

Abstract

A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.

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Year:  1991        PMID： 1682145     DOI： 10.1002/elps.1150120508

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  14 in total

1.  Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein.

Authors:  A L Kruckeberg; L Ye; J A Berden; K van Dam
Journal:  Biochem J       Date:  1999-04-15       Impact factor: 3.857

2.  Localization of a Nod factor-binding protein in legume roots and factors influencing its distribution and expression.

Authors:  G Kalsi; M E Etzler
Journal:  Plant Physiol       Date:  2000-11       Impact factor: 8.340

3.  Esa1p is an essential histone acetyltransferase required for cell cycle progression.

Authors:  A S Clarke; J E Lowell; S J Jacobson; L Pillus
Journal:  Mol Cell Biol       Date:  1999-04       Impact factor: 4.272

4.  Transcriptional activation of the interleukin-2 promoter by hepatitis C virus core protein.

Authors:  A Bergqvist; C M Rice
Journal:  J Virol       Date:  2001-01       Impact factor: 5.103

5.  The novel fission yeast protein Pal1p interacts with Hip1-related Sla2p/End4p and is involved in cellular morphogenesis.

Authors:  Wanzhong Ge; Ting Gang Chew; Volker Wachtler; Suniti N Naqvi; Mohan K Balasubramanian
Journal:  Mol Biol Cell       Date:  2005-06-22       Impact factor: 4.138

6.  The multiprotein exocyst complex is essential for cell separation in Schizosaccharomyces pombe.

Authors:  Hongyan Wang; Xie Tang; Jianhua Liu; Susanne Trautmann; David Balasundaram; Dannel McCollum; Mohan K Balasubramanian
Journal:  Mol Biol Cell       Date:  2002-02       Impact factor: 4.138

7.  Nine-month follow-up of the insulin receptor signalling cascade in the brain of streptozotocin rat model of sporadic Alzheimer's disease.

Authors:  J Osmanovic Barilar; A Knezovic; E Grünblatt; P Riederer; M Salkovic-Petrisic
Journal:  J Neural Transm (Vienna)       Date:  2014-12-12       Impact factor: 3.575

8.  The water- and salt-stress-regulated Asr1 (abscisic acid stress ripening) gene encodes a zinc-dependent DNA-binding protein.

Authors:  Yossi Kalifa; Ayelet Gilad; Zvia Konrad; Michele Zaccai; Pablo A Scolnik; Dudy Bar-Zvi
Journal:  Biochem J       Date:  2004-07-15       Impact factor: 3.857

9.  Creatine supplementation in health and disease. Effects of chronic creatine ingestion in vivo: down-regulation of the expression of creatine transporter isoforms in skeletal muscle.

Authors:  M L Guerrero-Ontiveros; T Wallimann
Journal:  Mol Cell Biochem       Date:  1998-07       Impact factor: 3.396

10.  Dufulin activates HrBP1 to produce antiviral responses in tobacco.

Authors:  Zhuo Chen; Mengjiao Zeng; Baoan Song; Chengrui Hou; Deyu Hu; Xiangyang Li; Zhenchao Wang; Huitao Fan; Liang Bi; Jiaju Liu; Dandan Yu; Linhong Jin; Song Yang
Journal:  PLoS One       Date:  2012-05-25       Impact factor: 3.240
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