Literature DB >> 16820974

Purification and characterization of two types of chitosanase from a Microbacterium sp.

Yuying Sun1, Wanshun Liu, Baoqin Han, Jiquan Zhang, Bing Liu.   

Abstract

Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products.

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Year:  2006        PMID： 16820974     DOI： 10.1007/s10529-006-9101-z

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  7 in total

1.  Bacterial diversity and structural changes of oyster shell during 1-year storage.

Authors:  Shah Md Asraful Islam; Sun Joo Hong; Kye Man Cho; Renukaradhya K Math; Jae Young Heo; Young Han Lee; Ki Sang Lee; Han Dae Yun
Journal:  Microb Ecol       Date:  2008-09-02       Impact factor: 4.552

2.  Chitinase production by Bacillus thuringiensis and Bacillus licheniformis: their potential in antifungal biocontrol.

Authors:  Eman Zakaria Gomaa
Journal:  J Microbiol       Date:  2012-02-27       Impact factor: 3.422

3.  Biochemical and molecular studies on the resistance mechanisms in tea [Camellia sinensis (L.) O. Kuntze] against blister blight disease.

Authors:  Sam Nirmala Nisha; Gajjeraman Prabu; Abul Kalam Azad Mandal
Journal:  Physiol Mol Biol Plants       Date:  2018-06-18

4.  Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp. in Escherichia coli.

Authors:  Yuying Sun; Jiquan Zhang; Shujun Wang
Journal:  Indian J Microbiol       Date:  2014-11-01       Impact factor: 2.461

5.  A Novel Chitosanase from Penicillium oxalicum M2 for Chitooligosaccharide Production: Purification, Identification and Characterization.

Authors:  Shining Cao; Pei Gao; Wenshui Xia; Shaoquan Liu; Bin Wang
Journal:  Mol Biotechnol       Date:  2022-03-09       Impact factor: 2.860

6.  Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5.

Authors:  Xu-Fen Zhu; Ying Zhou; Jun-Li Feng
Journal:  J Zhejiang Univ Sci B       Date:  2007-11       Impact factor: 3.066

7.  Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG.

Authors:  Pingping Zhang; Wei Zhou; Peng Wang; Li Wang; Mingli Tang
Journal:  Braz J Microbiol       Date:  2013-04-09       Impact factor: 2.476
  7 in total

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