Minna Laitinen1, Riitta Kivikari, Martti Hirn. 1. Department of Orthopedics and Traumatology, University Hospital of Tampere and Medical School, University of Tampere. Tampere, FI-33521. Finland. minna.laitinen@pshp.fi
Abstract
BACKGROUND: International standards indicate that bone allografts for human use should be stored in a -40 degrees C or colder environment and the storage time should be up to 5 years. Bone allografts contain lipids which oxidate and become toxic with time. MATERIAL AND METHODS: We determined lipid oxidation in femoral head bone allografts by headspace gas chromatography at different storage temperatures and storage times. RESULTS: We found that lipid oxidation in fresh-frozen bone allograft was influenced by storage temperature and storage time. Lipid oxidation was significantly more rapid at -30 degrees C than at -70 degrees C. Even at -70 degrees C, however, some oxidation occurred but it was quite minimal after 3 years. INTERPRETATION: Because of the negative effects of lipid oxidation, we recommend a storage temperature of -70 degrees C or lower for bone allografts.
BACKGROUND: International standards indicate that bone allografts for human use should be stored in a -40 degrees C or colder environment and the storage time should be up to 5 years. Bone allografts contain lipids which oxidate and become toxic with time. MATERIAL AND METHODS: We determined lipid oxidation in femoral head bone allografts by headspace gas chromatography at different storage temperatures and storage times. RESULTS: We found that lipid oxidation in fresh-frozen bone allograft was influenced by storage temperature and storage time. Lipid oxidation was significantly more rapid at -30 degrees C than at -70 degrees C. Even at -70 degrees C, however, some oxidation occurred but it was quite minimal after 3 years. INTERPRETATION: Because of the negative effects of lipid oxidation, we recommend a storage temperature of -70 degrees C or lower for bone allografts.
Authors: Jessica Martínez-Vargas; Laura Roqué; Irene Del Canto; José Carrillo-Ortiz; Carles Orta; Javier Quesada Journal: Naturwissenschaften Date: 2021-04-20
Authors: Alexandre Carneiro Bitar; Luiz Augusto Ubirajara Santos; Alberto Tesconi Croci; João Alberto Ramos Maradei Pereira; Edgard N França Bisneto; Arlete Mazzini Miranda Giovani; Claudia Regina G C M Oliveira Journal: Clinics (Sao Paulo) Date: 2010-03 Impact factor: 2.365